Tag

Part:BBa_K416003

Designed by: John Phair   Group: iGEM10_NYU   (2010-06-23)

Yeast Secretion Tag

This secretion tag can direct extracellular secretion of any protein fused to it when linked to the C-terminus of that protein. After targeting the fusion through the ER to the golgi, this "pre-pro" tag is cleaved by the KEX-2 protease in the trans golgi, resulting in secretion of protein without this tag attached. To use this tag, attached it directly to your proteins CDS.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/eukaryote/yeast
//proteindomain/localization
Parameters
None