Composite
Pc-OxyR

Part:BBa_K4225000

Designed by: Leon Ritchie Salim   Group: iGEM22_HKUST   (2022-10-07)


oxyR with constitutive promoter, RBS and 6xHis

OxyR protein (BBa_K1104200) that is controlled by a constitutive promoter(BBa_J23109) and RBS(BBa_B0034). A double terminator (BBa_B0015) is used to stop transcription process.

Contribution: HKUST 2022

This composite part is used by the HKUST 2022 team for two other composite parts, BBa_K4225003 and BBa_K4225006 to characterize oxySp, katGp and their interaction with oxyR. Result of the H2O2 assay of those composite parts can be seen in their respective composite part pages.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None