Frequently Used Parts
The iGEM Registry has a vast collection of parts that have been contributed over the course of our 10 year history. We have more than 20,000 parts that iGEM students (collegiate and high school), graduate students, postdocs, and PIs have contributed to the collection. Some of our parts have been used many, many times. In general, these are the older parts in our collection that have served as the foundation of many projects.
The job of every iGEMer and contributor to the Registry is to make parts users will want to use in coming years. With over 20,000 parts already in our collection, this is not an easy feat. You will need to create great parts for them to become among our most used.
We have many ribosome binding sites in our collection. You may want a strong RBS or one for your organism of choice. However, if you're not sure, you might want one that many other people have used, and will just work in
The purpose of this page is to showcase the most highly-used parts in our collection so you may see how iGEMers on different teams approach making great parts.
Top 10 Most Used Parts on the Registry
| Name | Description | Length | Created by | Uses | Status | Doc |
|---|---|---|---|---|---|---|
| BBa_B0034 | RBS (Elowitz 1999) -- defines RBS efficiency | 12 | Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. | 5428 | In stock | 25043 |
| BBa_B0015 | double terminator (B0010-B0012) | 129 | Reshma Shetty | 4772 | In stock | 12466 |
| BBa_R0040 | TetR repressible promoter | 54 | June Rhee, Connie Tao, Ty Thomson, Louis Waldman | 1081 | In stock | 25578 |
| BBa_B0010 | T1 from E. coli rrnB | 80 | Randy Rettberg | 1064 | In stock | 11502 |
| BBa_B0012 | TE from coliphageT7 | 41 | Reshma Shetty | 1021 | In stock | 5501 |
| BBa_B0030 | RBS.1 (strong) -- modified from R. Weiss | 15 | Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. | 1001 | In stock | 17415 |
| BBa_B0032 | RBS.3 (medium) -- derivative of BBa_0030 | 13 | Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. | 907 | In stock | 10737 |
| BBa_E0040 | green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212 | 720 | jcbraff | 875 | In stock | 35565 |
| BBa_R0010 | promoter (lacI regulated) | 200 | 843 | In stock | 46722 | |
| BBa_J23100 | constitutive promoter family member | 35 | John Anderson | 786 | In stock | 83466 |
Top 10 Most Used Coding Region Parts
| Name | Description | Length | Created by | Uses | Status | Doc |
|---|---|---|---|---|---|---|
| BBa_E0040 | green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212 | 720 | jcbraff | 875 | In stock | 35565 |
| BBa_E1010 | **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral) | 706 | Drew Endy | 593 | In stock | 46440 |
| BBa_C0040 | tetracycline repressor from transposon Tn10 (+LVA) | 685 | June Rhee, Connie Tao, Ty Thomson, Louis Waldman. | 318 | In stock | 12575 |
| BBa_C0051 | cI repressor from E. coli phage lambda (+LVA) | 775 | Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross | 295 | In stock | 7093 |
| BBa_C0012 | lacI repressor from E. coli (+LVA) | 1153 | Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton | 265 | In stock | 21550 |
| BBa_C0062 | luxR repressor/activator, (no LVA?) | 781 | Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr | 247 | In stock | 42298 |
| BBa_C0061 | autoinducer synthetase for AHL | 643 | Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr | 217 | In stock | 11645 |
| BBa_E0030 | enhanced yellow fluorescent protein derived from A. victoria GFP | 723 | Caitlin Conboy and Jennifer Braff | 140 | In stock | 8152 |
| BBa_I746916 | superfolder GFP coding sequence | 720 | Stefan Milde | 102 | In stock | 41280 |
| BBa_K592009 | amilCP, blue chromoprotein | 669 | Lei Sun | 99 | In stock | 47318 |
Top 10 Most Used Promoters
| Name | Description | Length | Created by | Uses | Status | Doc |
|---|---|---|---|---|---|---|
| BBa_R0040 | TetR repressible promoter | 54 | June Rhee, Connie Tao, Ty Thomson, Louis Waldman | 1081 | In stock | 25578 |
| BBa_R0010 | promoter (lacI regulated) | 200 | 843 | In stock | 46722 | |
| BBa_J23100 | constitutive promoter family member | 35 | John Anderson | 786 | In stock | 83466 |
| BBa_R0011 | Promoter (lacI regulated, lambda pL hybrid) | 55 | Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton | 598 | In stock | 23492 |
| BBa_I0500 | Inducible pBad/araC promoter | 1210 | Sri Kosuri | 484 | In stock | 48514 |
| BBa_J23119 | constitutive promoter family member | 35 | John Anderson | 442 | In stock | 31843 |
| BBa_R0062 | Promoter (luxR & HSL regulated -- lux pR) | 55 | Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr | 374 | In stock | 77850 |
| BBa_J23101 | constitutive promoter family member | 35 | John Anderson | 326 | In stock | 53848 |
| BBa_R0051 | promoter (lambda cI regulated) | 49 | Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross | 284 | In stock | 9224 |
| BBa_I719005 | T7 Promoter | 23 | Imperial 2007 | 262 | In stock | 7912 |
Top 10 Most Used Ribosome Binding Sites
| Name | Description | Length | Created by | Uses | Status | Doc |
|---|---|---|---|---|---|---|
| BBa_B0034 | RBS (Elowitz 1999) -- defines RBS efficiency | 12 | Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. | 5428 | In stock | 25043 |
| BBa_B0030 | RBS.1 (strong) -- modified from R. Weiss | 15 | Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. | 1001 | In stock | 17415 |
| BBa_B0032 | RBS.3 (medium) -- derivative of BBa_0030 | 13 | Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. | 907 | In stock | 10737 |
| BBa_B0031 | RBS.2 (weak) -- derivative of BBa_0030 | 14 | Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. | 218 | In stock | 13140 |
| BBa_J61100 | Ribosome Binding Site Family Member | 12 | John Anderson | 184 | In stock | 8254 |
| BBa_Z0262 | Medium strength T7.2 RBS | 20 | Sriram Kosuri | 127 | Not in stock | 3181 |
| BBa_J61101 | Ribosome Binding Site Family Member | 12 | John Anderson | 126 | In stock | 6164 |
| BBa_Z0261 | Strong T7.2 RBS | 20 | Sriram Kosuri | 98 | Not in stock | 3179 |
| BBa_K2066527 | B0034 with scar TACTAG | 18 | Joseph L Maniaci | 72 | Not in stock | 3316 |
| BBa_K2406216 | MoClo RBS B0034 | 21 | Jack T. Suitor | 63 | Not in stock | 1422 |
Top 10 Most Used Terminators
| Name | Description | Length | Created by | Uses | Status | Doc |
|---|---|---|---|---|---|---|
| BBa_B0015 | double terminator (B0010-B0012) | 129 | Reshma Shetty | 4772 | In stock | 12466 |
| BBa_B0010 | T1 from E. coli rrnB | 80 | Randy Rettberg | 1064 | In stock | 11502 |
| BBa_B0012 | TE from coliphageT7 | 41 | Reshma Shetty | 1021 | In stock | 5501 |
| BBa_B0014 | double terminator (B0012-B0011) | 95 | Reshma Shetty | 412 | In stock | 4662 |
| BBa_B1006 | Terminator (artificial, large, %T~>90) | 39 | Haiyao Huang | 221 | In stock | 1859 |
| BBa_K731721 | T7 terminator | 48 | Giacomo Giacomelli, Anna Depetris | 114 | In stock | 8059 |
| BBa_B0025 | double terminator (B0015), reversed | 129 | Caitlin Conboy | 102 | In stock | 4033 |
| BBa_B0011 | LuxICDABEG (+/-) | 46 | Reshma Shetty | 94 | In stock | 4374 |
| BBa_K2933030 | T7 terminator | 48 | Jiahao Xie | 80 | In stock | 1226 |
| BBa_K2486006 | ECK120029600 - Escherichia coli K-12 terminator | 90 | Cau� Westmann and Gabriel Lencioni Lovate | 77 | Not in stock | 2080 |