Plasmid Backbone Overview

Why would you want to use more than one plasmid backbone?

One part can be used in several different plasmids backbones. It's easy to move a part from one plasmid backbone to another.

Use any assembly method you want!

You may have a plasmid backbone that works well with your assembly method (and standard)
Just make sure that your parts are BioBrick compatible when it's time to ship them in pSB1C3 to the Registry

Operate your part in the plasmid backbone that works for your system

Maybe your part uses a lot of cellular resources, so you'll want to operate it in a low copy plasmid backbone
Or you want to test your part in different organisms by using a multi-host plasmid backbone

Use a plasmid backbone to measure your part

You may want to use a plasmid backbone that will help you measure attributes of your part
Have a library of promoters you want to test? Use a plasmid backbone with a built in coding region.
Don't forget, documenting and characterizing your part is important!

After you're done assembling, operating, and measuring your part, ship it to the Registry in pSB1C3

Shipping in pSB1C3 lets the Registry maintain and test all parts in the same way
Anyone who wants to use your part can easily and reliably move it into another plasmid backbone

the part does not include the prefix and suffix
the plasmid backbone is defined as the sequence starting with the suffix and ending at the prefix

Examples

The sequence of a coding region part would start with the start codon and end with the stop codon
The sequence of the pSB1C3 plasmid backbone starts with the suffix and ends with the prefix

Plasmid Backbones: Overview | Shipping in pSB1C3 | Features | Linearized Plasmid Backbones | Plasmid Backbone Catalog