RBS

Part:BBa_K4169023

Designed by: Jie Min   Group: iGEM22_HZAU-China   (2022-09-30)


a theophylline-inducible riboswitch

This a theophylline-inducible riboswitch which works at the translation level, and the riboswitch produce low levels of background gene expression in the absence of the small molecule theophylline and large (>50-fold) increases in expression in the presence of the ligand.

Usage and Biology

This is a switch regulated by theophylline at the translation level, that is, a certain concentration of theophylline molecules in the outside world can combine with this riboswitch, and the subsequent translation process of ribosomes can be carried out through conformational changes.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 4
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None