Coding

Part:BBa_K1583105:Design

Designed by: Stefan Robert Marsden   Group: iGEM15_TU_Delft   (2015-09-04)


pRha + CsgA_Mfp3_His fusion protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A mutation in CsgA had to be introduced to remove a PstI restriction site. This was done without changing the protein sequence and a codon optimizer was used to ensure high expression in E.coli.


Source

This DNA was synthesized. The design was based on the paper "Strong underwater adhesives made by self-assembling multi-protein nanofibres".[1] 1. C.Zhong, T.Gurry, A.Cheng, J.Downey, Z.Deng, C. Stultz, T.Lu, Nature Nanotechnology, 2014, 9, 858-866.

The CsgA sequence originates from the genomic DNA of E. coli K-12 MG1655 (http://www.ncbi.nlm.nih.gov/gene/949055).

References