![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K1583105:Design
Designed by: Stefan Robert Marsden Group: iGEM15_TU_Delft (2015-09-04)
pRha + CsgA_Mfp3_His fusion protein
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
A mutation in CsgA had to be introduced to remove a PstI restriction site. This was done without changing the protein sequence and a codon optimizer was used to ensure high expression in E.coli.
Source
This DNA was synthesized. The design was based on the paper "Strong underwater adhesives made by self-assembling multi-protein nanofibres".[1] 1. C.Zhong, T.Gurry, A.Cheng, J.Downey, Z.Deng, C. Stultz, T.Lu, Nature Nanotechnology, 2014, 9, 858-866.
The CsgA sequence originates from the genomic DNA of E. coli K-12 MG1655 (http://www.ncbi.nlm.nih.gov/gene/949055).