Difference between revisions of "Promoters/Catalog/Constitutive"

Revision as of 18:50, 17 April 2009

Constitutive E. coli promoters

All the promoters in this section are E. coli promoters that are constitutive meaning that their activity is dependent on the availability of RNA polymerase holoenzyme, but is not affected by any transcription factors. If you find any promoters on this page that you know to be regulated by a particular transcription factor, please let us know, or re-categorize the part yourself!

Constitutive E. coli σ⁷⁰ promoters

This section lists promoters that are recognized by E. coli σ⁷⁰ RNAP. σ⁷⁰ is the major E. coli sigma factor so there should be RNAP present to transcribe these promoters under most growth conditions (although maximally during exponential growth).

Constitutive E. coli σ^S promoters

This section lists promoters that are recognized by E. coli σ^S RNAP. σ^S is the major stationary phase E. coli sigma factor. Use these promoters when you want high promoter activity during stationary phase or during starvation. Since σ^S promoters have the same consensus promoter sequence as σ⁷⁰ promoters, you may find these promoters will be weakly expressed by σ⁷⁰ RNAP.

Constitutive E. coli σ³² promoters

This section lists promoters that are recognized by E. coli σ³² RNAP. σ³² is the major heat shock sigma factor in E. coli. Use these promoters when you want high promoter activity during heat shock. These promoters are also active under several different shock responses.

Constitutive E. coli σ⁵⁴ promoters

This section lists promoters that are recognized by E. coli σ⁵⁴ RNAP. σ⁵⁴ is a minor E. coli sigma factor that is most highly expressed during nitrogen-limitation. Use these promoters if you want promoter activity to depend on ammonia or nitrogen levels. Alternatively, since this is a minor sigma factor that recognizes promoters with a very different sequence to σ⁷⁰ promoters, this sigma factor could be repurposed to be be expressed and active during some user-chosen set of environmental conditions, essentially producing a user controllable RNAP.

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Constitutive B. subtilis σ^A promoters

This section lists promoters that are recognized by B. subtilis σ^A RNAP. σ^A is the major B. subtilis sigma factor so there should be RNAP present to transcribe these promoters under most growth conditions (although maximally during exponential growth).

Constitutive B. subtilis σ^B promoters

This section lists promoters that are recognized by B. subtilis σ^B RNAP. σ^B is the major stationary phase E. coli sigma factor. Use these promoters when you want high promoter activity during stationary phase or during starvation.

Constitutive promoters from miscellaneous prokaryotes

The promoters in this table are prokaryotic promoters that are constitutive meaning that the activity of these promoters is dependent only on the concentration of the appropriate RNA polymerase and sigma factor. Constitutive prokaryotic promoters other than those from E. coli or B. subtilis are listed here. The equivalent pages for E. coli and B. subtilis are listed here and here. Note that many prokaryotic RNA polymerases recognize similar promoter sequences so many of these promoter sequences will work in other prokaryotes.

Constitutive promoters from bacteriophage T7

These promoters are recognized by the T7 RNA Polymerase and are constitutive meaning that their activity is dependent on the availability of RNA polymerase, but is not affected by any transcription factors. T7 promoters are known to have high activity levels and are also highly orthogonal to bacterial promoters since the respective RNA Polymerases are unrelated.

Constitutive promoters from bacteriophage SP6

All the promoters in this table are recognized by the SP6 RNA Polymerase and are constitutive meaning that their activity is dependent on the availability of RNA polymerase, but is not affected by any transcription factors. SP6 promoters are known to have high activity levels and are also highly orthogonal to bacterial promoters since the respective RNA Polymerases are unrelated.

Constitutive promoters from yeast

All the promoters in this table are yeast promoters that are constitutive meaning that their activity is dependent on the availability of RNA polymerase holoenzyme, but is not affected by any transcriptional regulators.

Constitutive promoters from miscellaneous eukaryotes

All the promoters in the table below are eukaryotic promoters that are constitutive meaning that their activity is dependent on the availability of RNA polymerase holoenzyme, but is not affected by any transcription factors.