Regulatory

Part:BBa_M31201

Designed by: Katherine Aull   Group: BE.109 Instructors BE.109 2006 MIT 20.109 Spring07 MIT 20.109 Fall07   (2008-05-14)

Yeast CLB1 promoter region, G2/M cell cycle specific

Description of CLB1 (from SGD): B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome.

More information found here: http://db.yeastgenome.org/cgi-bin/locus.pl?locus=CLB1

Part contains 500 base pairs upstream of the ORF. Mutation at position 131, G->A, to eliminate Pst1 site. Is NOT a minimal promoter and has not been tested for composability.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 423
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//rnap/eukaryote/yeast
//direction/forward
//chassis/eukaryote/yeast
//promoter
//regulation/constitutive
Parameters
negative_regulators
positive_regulators