Regulatory

Part:BBa_Z0252

Designed by: T7.2   Group: T7.2   (2005-04-05)


T7 weak binding and processivity

Promoter for T7 RNA polymerase


Usage and Biology

Taken from phi3.8 region of from wild-type T7. Region includes from -22 to +13 relative to the start of transcription in order to be consistant with strong promoter size. Phi3.8 was chosen over other promoter sequences because of more data on relative strength than the other T7 weak promoters.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 25


[edit]
Categories
//rnap/bacteriophage/T7
//direction/forward
//chassis/prokaryote/ecoli
//promoter
//regulation/constitutive
//classic/regulatory/uncategorized
//chassis/bacteriophage/T7
Parameters
negative_regulators
positive_regulators