Regulatory

Part:BBa_Z0251

Designed by: T7.2   Group: T7.2   (2005-04-05)


T7 strong promoter

Promoter for T7 RNA polymerase


Usage and Biology

Taken from phi10 region of from wild-type T7. Region includes from -22 to +13 relative to the start of transcription. The left boundary was chosen due to homology found on strong T7 promoters. The right boundary was chosen due to evidence for sequence dependence until initial bubble collapse (Gong et al., 2004)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 25


[edit]
Categories
//rnap/bacteriophage/T7
//direction/forward
//chassis/prokaryote/ecoli
//promoter
//regulation/constitutive
//classic/regulatory/uncategorized
//chassis/bacteriophage/T7
Parameters
negative_regulators
positive_regulators