Difference between revisions of "Test2"

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=Photoproteins=  
 
=Photoproteins=  
 
(Aequorin, Obelin
 
(Aequorin, Obelin

Revision as of 22:53, 6 March 2013

Icon Sensors.png


Biosafety: Parts improving biological safety, containment.
Biosynthesis: Parts involved in the production of chemicals and metabolites are listed here.
Biodegradation: Parts involved in the degradation of chemicals are listed here.
Cell-cell signaling and quorum sensing: Parts involved in intercellular signaling and quorum sensing between bacteria.
Cell death: Parts involved in killing cells.
Coliroid: Parts involved in taking a bacterial photograph.
Conjugation: Parts involved in DNA conjugation between bacteria.
DNA binding: Parts involved in xxx.
DNA recombination: Parts involved in DNA recombination are listet here.
Ions: Parts involved in sensing, transport and binding of different ions are listet in here.
Ligand Binding: Parts involved in binding chemical structures are listet here.
Light production and sensing: Parts involved in generation and sensing of light are listet here.
Motility and chemotaxis: Parts involved in motility or chemotaxis of cells are listet here.
Odor production and sensing: Parts that produce or sense odorants aer listet here.
Sensors: Parts used as biosensors are listed here.
Viral vectors: Parts involved in the production and modification of Viral vectors are listet here..












Fluorescent Proteins

Featured Parts, [http://www.microscopyu.com/articles/livecellimaging/fpintro.html Nikkon], [http://2012.igem.org/Team:Uppsala_University/Chromoproteins Chromoproteins Uppsala] https://parts.igem.org/Part:BBa_K180009 mBanana

Name Exitation [nm] Emmission [nm] Quant. yield Molar extinct. BioBrick RFC10 BioBrick RFC25 Reference
Cerulean 433 475 0.62 43000 BBa_E2020
GFPmut3b 501 511 BBa_E0040
amilGFP 503 512 0.67 75200 BBa_K592010 [http://www.ncbi.nlm.nih.gov/nuccore/ay646067 GeneBank]
mVenus 515 528 0.57 92200 BBa_I757008
mOrange 548 562 0.69 71000 BBa_E2050
mStrawbery 574 596 0.29 90000 BBa_K399001
mRFP1 584 607 0.25 50000 BBa_E1010
mCherry 587 610 0.22 72000 BBa_E2060
eforRed 589 609 0.16 111300 BBa_K592012 [http://www.ncbi.nlm.nih.gov/nuccore/EU498726 GeneBank]
cjBlue 610 BBa_K592011 [http://www.ncbi.nlm.nih.gov/pubmed/17028187 PubMed]
aeBlue BBa_K864401 [http://www.ncbi.nlm.nih.gov/pubmed/16164420 PubMed]
amilCP 588 NA NA 8760 BBa_K592009 [http://www.ncbi.nlm.nih.gov/nuccore/AY646075 GeneBank]



More...
NameTypeDescriptionLengthStatus
BBa_K125502CodingphaB BioPlastic polyhydroxybutyrate synthesis pathway (origin PCC6803 slr1993)726In stock
BBa_K759005CodingphaB (acetoacetyl-CoA reductase orgin from Ralstonia eutropha)738In stock
BBa_K759007Translational_UnitRBS(B0034)and phaB (acetoacetyl-CoA reductase orgin from Ralstonia eutropha)756In stock
BBa_K125501CodingphaA BioPlastic polyhydroxybutyrate synthesis pathway (origin PCC6803 slr1994)1233It's complicated
BBa_K125503CodingphaC BioPlastic polyhydroxybutyrate synthesis pathway (origin PCC6803 slr1830) 1140It's complicated
BBa_K125504CodingphaE BioPlastic polyhydroxybutyrate synthesis pathway (origin PCC6803 slr1829) 996It's complicated
BBa_K125801Translational_UnitRBS-phaA1251It's complicated
BBa_K125803Translational_UnitRBS-phaC1158It's complicated
BBa_K125804Translational_UnitRBS-phaE1014It's complicated
BBa_K156012CodingphaA (acetyl-CoA acetyltransferase)1182It's complicated
BBa_K156013CodingphaB1 (acetyacetyl-CoA reductase)741It's complicated
BBa_K156014CodingphaC1 (Poly(3-hydroxybutyrate) polymerase)1349It's complicated
BBa_K156015Translational_UnitRBS + phaA1201It's complicated
BBa_K156016Translational_UnitRBS + phaB1760It's complicated
BBa_K156021GeneratorPromoter + RBS + phaA + double terminator1400It's complicated
BBa_K156022GeneratorPromoter + RBS + phaB1 + double terminator959It's complicated
BBa_K156031GeneratorRBS + phaA + double terminator1338It's complicated
BBa_K156033GeneratorRBS + phaB1 + double terminator897It's complicated
BBa_K338003CompositePHA Synthase Composite, Part 1/21303It's complicated
BBa_K338004CompositePHA Synthase Composite, Part 2/22253It's complicated
BBa_K338023CompositePlastic Intermediate: phaB1 + RBS761It's complicated
BBa_K338022CompositePlastic Intermediate: phaA + RBS1202It's complicated
BBa_K338024CompositePlastic Intermediate: phaC1 + Terminator1486It's complicated
BBa_K759004CodingphaA (beta-ketothiolase orgin from Ralstonia eutropha)1182It's complicated
BBa_K759006Translational_UnitB0034 and phaA (beta-ketothiolase orgin from Ralstonia eutropha)1200It's complicated
BBa_K759008Translational_UnitB0034 and phaC (BBa_K342001)1809It's complicated
BBa_K759009Translational_UnitRBS-phaC-RBS-phaA-RBS-phaB3781It's complicated
BBa_K759010CompositeP3HB producing construct (without a promoter)3918It's complicated
BBa_K759011CompositeP3HB producing construct 3980It's complicated
BBa_K2783010CodingPhenylalanine ammonia lyase 22154It's complicated
BBa_K125802Translational_UnitRBS-phaB744Not in stock
BBa_K156018CompositePromoter + RBS + phaA1263Not in stock

Luciferases

Name Organism Substrate pH optimum Amino acids Mol. mass [kDa] Emmission [nm] BioBrick RFC10 BioBrick RFC25 Reference
Photinus/Firefly luciferase D-luciferin + ATP 550 60.6 578 BBa_I712019
Renilla luciferase coelenterazine + O2 311 36.0 480 BBa_J52008
Gaussia luciferase coelenterazine + O2 189 20.3 480 BBa_K509005
Nano luciferase furimazine 171 19.1 460 [http://www.reportergene.com/2013/01/the-nanoluc-luciferase-reporter-dossier.html 1]


More...
NameDescriptionAuthorLength
BBa_K404001[AAV2]-Rep-VP123Team Freiburg 20104214
BBa_K404002[AAV2]-Rep-VP123_p5-TATAlessFreiburg Bioware 20104386
BBa_K404003[AAV2]-Rep-VP123(ViralBrick-587KO-empty)_p5-TATAlessFreiburg Bioware 20104386
BBa_K404004[AAV2]-Rep-VP13(ViralBrick-587KO-empty)_p5-TATAlessFreiburg Bioware 20104402
BBa_K404005[AAV2]-Rep-VP23(ViralBrick-587KO-empty)_p5-TATAlessFreiburg Bioware 20104402
BBa_K404006[AAV2]-VP123Freiburg Bioware 20102208
BBa_K404007pCMV_[AAV2]-VP123Freiburg Bioware 20102998
BBa_K404008[AAV2]-VP1Freiburg Bioware 20102202
BBa_K404009pCMV_[AAV2]-VP1Freiburg Bioware 20102910
BBa_K404010[AAV2]-VP2Freiburg Bioware 20101830
BBa_K404011pCMV_[AAV2]-VP2Freiburg Bioware 20102500
BBa_K404012[AAV2]-VP3Freiburg Bioware 20101635
BBa_K404013pCMV_[AAV2]-VP3Freiburg Bioware 20102305
BBa_K404090[AAV2]-Rep40ex Freiburg Bioware 2010940
BBa_K404092[AAV2]-Rep68ex Freiburg Bioware 20101612
BBa_K404094[AAV2]-AAPex Freiburg Bioware 2010616
BBa_K404100[AAV2]-left-ITRFreiburg Bioware 2010147
BBa_K404101[AAV2]-right-ITR Freiburg Bioware 2010138
BBa_K404103p5 promoterFreiburg Bioware 2010166
BBa_K404104p5-TATAless promoterFreiburg Bioware 2010164
BBa_K404105p40 promoterFreiburg Bioware 2010182
BBa_K404107Beta-Globin-IntronFreiburg Bioware 2010495
BBa_K404108hGH terminatorFreiburg Bioware 2010481
BBa_K404114[AAV2]-left-ITR_pCMV Freiburg Bioware 2010809
BBa_K404115[AAV2]-left-ITR_phTERT Freiburg Bioware 2010612
BBa_K404116hGH_[AAV2]-right-ITR Freiburg Bioware 2010632
BBa_K404117[AAV2]-left-ITR_pCMV_betaglobin Freiburg Bioware 20101310
BBa_K404118[AAV2]-left-ITR_phTERT_betaglobin Freiburg Bioware 20101113
BBa_K404119[AAV2]-left-ITR_pCMV_betaglobin_mVenus_hGH_[AAV2]-right-ITR Freiburg Bioware 20102687
BBa_K404120[AAV2]-left-ITR_pCMV_betaglobin_CFP_hGH_[AAV2]-right-ITR Freiburg Bioware 20102687
BBa_K404121[AAV2]-left-ITR_pCMV_betaglobin_mCherry_hGH_[AAV2]-right-ITR Freiburg Bioware 20102826
BBa_K404122[AAV2]-left-ITR_pCMV_betaglobin_mGMK_TK30_hGH_[AAV2]-right-ITR Freiburg Bioware 20103659
BBa_K404123[AAV2]-left-ITR_phTERT_betaglobin_mVenus_hGH_[AAV2]-right-ITR Freiburg Bioware 20102490
BBa_K404124[AAV2]-left-ITR_phTERT_betaglobin_mGMK_TK30_hGH_[AAV2]-right-ITR Freiburg Bioware 20103462
BBa_K404125[AAV2]-left-ITR_phTERT_betaglobin_CD_hGH_[AAV2]-right-ITR Freiburg Bioware 20103057
BBa_K404126[AAV2]-left-ITR_pCMV_betaglobin_CD_hGH_[AAV2]-right-ITR Freiburg Bioware 20103254
BBa_K404127[AAV2]-left-ITR_pCMV_betaglobin_mVenus_[AAV2]-right-ITR Freiburg Bioware 20102200
BBa_K404128[AAV2]-left-ITR_pCMV_mVenus_hGH_[AAV2]-right-ITR Freiburg Bioware 20102190
BBa_K404129[AAV2]-left-ITR_phTERT_betaglobin_mCherry_hGH_[AAV2]-right-ITR Freiburg Bioware 20102629
BBa_K404150[AAV2]-VP23 Freiburg Bioware 20101917
BBa_K404151[AAV2]-VP23 (ViralBrick-587KO-Empty) Freiburg Bioware 20101917
BBa_K404152[AAV2]-VP1up Freiburg Bioware 2010411
BBa_K404153[AAV2]-NLS Freiburg Bioware 201021
BBa_K404154pCMV_Z-EGFR-1907_[AAV2]-VP23 (ViralBrick-587KO-Empty) Freiburg Bioware 20102767
BBa_K404155pCMV_Z-EGFR-1907_Short-Linker_[AAV2]-VP23 (ViralBrick-587KO-Empty) Freiburg Bioware 20102785
BBa_K404156pCMV_Z-EGFR-1907_Middle-Linker_[AAV2]-VP23 (ViralBrick-587KO-Empty) Freiburg Bioware 20102797
BBa_K404157pCMV_Z-EGFR-1907_Long-Linker_[AAV2]-VP23 (ViralBrick-587KO-Empty) Freiburg Bioware 20102809
BBa_K404158pCMV_Z-EGFR-1907_SEG-Linker_[AAV2]-VP23 (ViralBrick-587KO-Empty) Freiburg Bioware 20102881
BBa_K404159pCMV_CFP_Middle-Linker_[AAV2]-VP23 (ViralBrick-587KO-Empty) Freiburg Bioware 20103337
BBa_K404160pCMV_His-Tag_Middle-Linker_[AAV2]-VP23 (ViralBrick-587KO-Empty) Freiburg Bioware 20102641
BBa_K404161pCMV_DARPin-E01_Middle-Linker_[AAV2]-VP23 (ViralBrick-587KO-Empty) Freiburg Bioware 20103082
BBa_K404162pCMV_Z-EGFR-1907_Middle-Linker_[AAV2]-VP23 (ViralBrick-587KO-BAP) Freiburg Bioware 20102857
BBa_K404163pCMV_Z-EGFR-1907_Middle-Linker_[AAV2]-VP23 (ViralBrick-587KO-His-Tag) Freiburg Bioware 20102830
BBa_K404164pCMV_VP1up_NLS_Z-EGFR-1907_[AAV2]-VP23 (ViralBrick-587KO-Empty) Freiburg Bioware 20103211
BBa_K404165pCMV_VP1up_NLS_mVenus_[AAV2]-VP23 (ViralBrick-587KO-Empty) Freiburg Bioware 20103751
BBa_K404166pCMV_VP1up_NLS_His-Tag_[AAV2]-VP23 (ViralBrick-587KO-Empty) Freiburg Bioware 20103056
BBa_K404167pCMV_VP1up_NLS_mVenus_VP23(ViralBrick-587-KO-BAP)Freiburg Bioware 20103811
BBa_K404168pCMV_VP1up_NLS_mVenus_VP2/3(ViralBrick-587-KO-His)Freiburg Bioware 20103784
BBa_K404201ViralBrick-453-BAP Freiburg Bioware 2010114
BBa_K404202ViralBrick-453-His-Tag Freiburg Bioware 201087
BBa_K404203ViralBrick-453-RGD Freiburg Bioware 201096
BBa_K404204ViralBrick-453-Z34C Freiburg Bioware 2010165
BBa_K404205ViralBrick-587-BAP Freiburg Bioware 2010132
BBa_K404206ViralBrick-587-His-Tag Freiburg Bioware 2010105
BBa_K404207ViralBrick-587-RGD Freiburg Bioware 2010114
BBa_K404208ViralBrick-587-Beta Lactamase (BLA) Freiburg Bioware 2010174
BBa_K404209ViralBrick-587KO-BAPFreiburg Bioware 2010132
BBa_K404210ViralBrick-587KO-Empty Freiburg Bioware 201072
BBa_K404211ViralBrick-587KO-His-Tag Freiburg Bioware 2010105
BBa_K404212ViralBrick-587KO-RGD Freiburg Bioware 2010114
BBa_K404213ViralBrick-587KO-Z34C Freiburg Bioware 2010174
BBa_K404214ViralBrick-587KO-Z34C-SpacerFreiburg Bioware 2010189
BBa_K404222[AAV2]-Rep-VP123_P5-TATAless (ViralBrick-453-His-Tag) Freiburg Bioware 20104410
BBa_K404223[AAV2]-Rep-VP123_p5-TATAless (ViralBrick-453-RGD) Freiburg Bioware 20104419
BBa_K404224[AAV2]-Rep-VP123_P5-TATAless (ViralBrick-453-Z34C) Freiburg Bioware 20104488
BBa_K404225[AAV2]-Rep-VP123_P5-TATAless (ViralBrick-587-BAP) Freiburg Bioware 20104446
BBa_K404227[AAV2]-Rep-VP123_P5-TATAless (ViralBrick-587-RGD) Freiburg Bioware 20104428
BBa_K404229[AAV2]-Rep-VP123_P5-TATAless (ViralBrick-587KO-BAP) Freiburg Bioware 20104446
BBa_K404232[AAV2]-Rep-VP123_P5-TATAless (ViralBrick-587KO-RGD) Freiburg Bioware 20104428
BBa_K404234[AAV2]-Rep-VP123_P5-TATAless (ViralBrick-587KO-Z34C-Spacer) Freiburg Bioware 20104503
BBa_K404235[AAV2]Rep-VP123_p5TATAless (ViralBrick 453 Z34C-587 HSPG-KO)Freiburg Bioware 20104488
BBa_K404236[AAV2]Rep-VP123_p5TATAless (ViralBrick 453 RGD-587 HSPG-KO)Freiburg Bioware 20104419
BBa_K404241pCMV_[AAV2]VP123 (ViralBrick-453-BAP) Freiburg Bioware 20103049
BBa_K404242pCMV_[AAV2]VP123 (ViralBrick-453-His-Tag)Freiburg Bioware 20103022
BBa_K404243pCMV_[AAV2]VP123 (ViralBrick-453-RGD)Freiburg Bioware 20103031
BBa_K404244pCMV_[AAV2]VP123 (ViralBrick-453-Z34C) Freiburg Bioware 20103100
BBa_K404246pCMV_[AAV2]-VP123ex (ViralBrick-587-BAP)Freiburg Bioware 20103058
BBa_K404247pCMV_[AAV2]-VP123ex (ViralBrick-587-His-Tag)Freiburg Bioware 20103031
BBa_K404248pCMV_[AAV2]-VP123ex (ViralBrick-587-RGD)Freiburg Bioware 20103040
BBa_K404250pCMV_[AAV2]-VP123ex (ViralBrick-587-Beta-lactamase)Freiburg Bioware 20103787
BBa_K404251pCMV_[AAV2]-VP123ex (ViralBrick-587KO-BAP)Freiburg Bioware 20103058
BBa_K404252pCMV_[AAV2]-VP123ex (ViralBrick-587KO-Empty)Freiburg Bioware 20102998
BBa_K404253pCMV_[AAV2]-VP123ex (ViralBrick-587KO-His-Tag)Freiburg Bioware 20103031
BBa_K404254pCMV_[AAV2]-VP123ex (ViralBrick-587KO-RGD)Freiburg Bioware 20103040
BBa_K404255pCMV_[AAV2]-VP123ex (ViralBrick-587KO-Z34C)Freiburg Bioware 20103100
BBa_K404256pCMV_[AAV2]-VP123ex (ViralBrick-587KO-Z34C-Spacer)Freiburg Bioware 20103115
BBa_K404302Z-EGFR-1907 Freiburg Bioware 2010174
BBa_K404303Z-EGFR-1907_Short-Linker Freiburg Bioware 2010192
BBa_K404304Z-EGFR-1907_Middle-Linker Freiburg Bioware 2010204
BBa_K404305Z-EGFR-1907_Long-Linker Freiburg Bioware 2010216
BBa_K404306Z-EGFR-1907_SEG-Linker Freiburg Bioware 2010288
BBa_K404314DARPin-E01 Freiburg Bioware 2010459
BBa_K883001CCMV wt coat protein under IPTG induced promoterJeroen Bosman799

Photoproteins

(Aequorin, Obelin