Cell death
Parts that are involved in cell lysis and death are listed here.
Protein coding sequences
Note that several of the protein coding sequences listed here adhere to the Berkeley standard for physical composition.
| Name | Protein | Description | Tag | Direction | UniProt | KEGG | Length | Status |
|---|---|---|---|---|---|---|---|---|
| BBa_K112000 | Holin | T4 holin, complete CDS, berkeley standard | 657 | In stock | ||||
| BBa_K117000 | Lysis gene (promotes lysis in colicin-producing bacteria strain) | 144 | In stock | |||||
| BBa_K112306 | {lambda holin!} in BBb format | 318 | In stock | |||||
| BBa_K112806 | [T4 endolysin] | 514 | In stock | |||||
| BBa_K748002 | Truncated lysostaphin coding sequence. Lysostaphin has has a specific lytic action against S.aureus. | 741 | In stock | |||||
| BBa_K112002 | Holin | T4 holin, without stop codon, berkeley standard | 654 | It's complicated | ||||
| BBa_K112004 | a~T4 holin! in BBb | 661 | It's complicated | |||||
| BBa_K112006 | T4 antiholin! in BBb | 294 | It's complicated | |||||
| BBa_K112009 | 288 | It's complicated | ||||||
| BBa_K112010 | a~T4 antiholin! in BBb | 298 | It's complicated | |||||
| BBa_K112012 | T4 lysozyme! in BBb | 495 | It's complicated | |||||
| BBa_K112015 | 489 | It's complicated | ||||||
| BBa_K112016 | a~T4 lysozyme! in BBb | 499 | It's complicated | |||||
| BBa_K124014 | Bacteriophage Holin Gene pS105 | 317 | It's complicated | |||||
| BBa_K108001 | SRRz | 1242 | It's complicated | |||||
| BBa_K112300 | {lambda lysozyme!} in BBb format | 477 | It's complicated | |||||
| BBa_K112304 | {a~lambda lysozyme!} in BBb format | 481 | It's complicated | |||||
| BBa_K112310 | {a~lambda holin!}; adheres to Berkeley standard | 322 | It's complicated | |||||
| BBa_K112312 | {lambda antiholin!}; adheres to Berkeley standard | 324 | It's complicated | |||||
| BBa_K112316 | {a~lambda antiholin!}; adheres to Berkeley standard | 328 | It's complicated | |||||
| BBa_K124017 | Bacteriophage Lysis Cassette S105, R, and Rz | 1257 | It's complicated | |||||
| BBa_K284001 | Lysozyme from Gallus gallus | 539 | It's complicated | |||||
| BBa_K732003 | bbp9 - Bordetella phage lysin | 1527 | It's complicated | |||||
| BBa_K1639006 | PotB59-pomA | 1733 | It's complicated | |||||
| BBa_K1904000 | Mutated endolysin specific for Acinetobacter baumannii. | 558 | It's complicated | |||||
| BBa_K2812004 | Coding sequence for trunctated Lysostaphin fused to His-tagged HlyA | 1458 | It's complicated | |||||
| BBa_K2812006 | Coding sequence for Pyocin S5 with HlyA and His6-tag | 2217 | It's complicated | |||||
| BBa_K2685005 | φX174E | 276 | It's complicated | |||||
| BBa_K4321000 | Cytotoxic Protein 2Ba (Cyt2Ba) | 792 | ||||||
| BBa_K4133012 | LysKB317 (Endolysin) for Lactobacillus casei | 894 | ||||||
| BBa_K4706007 | BCL-2-associated athanogene 6 | AtBAG6 induced S. cerevisae cell death | none | forward | At2g46240 | 405 | ||
| BBa_K2812001 | Coding sequence for trunctated Lysostaphin | 738 | Not in stock | |||||
| BBa_K3317053 | NucD | 540 | Not in stock | |||||
| BBa_K3378000 | Chimeric lysin ClyR with activity against Streptococcus mutans. | 765 | Not in stock | |||||
| BBa_K3425036 | T7 lysozyme | T7 lysozyme for Type IIS standard (RFC1000) | forward | 453 | Not in stock |
 |
Nguyen Xuan Hung and the [http://2008.igem.org/Team:NTU-Singapore 2008 NTU Singapore iGEM team] designed and constructed the lysis protein coding sequence BBa_K117000. |  |
Katherine Jacobs and the [http://2008.igem.org/Team:Brown 2008 Brown University iGEM team] designed and constructed the lysis protein coding sequence BBa_K124014. |
 |
 |
Bing Xia, Aron Lau and the [http://2008.igem.org/Team:UC_Berkeley 2008 UC Berkeley iGEM team] designed and constructed many of the holin and antiholin protein coding sequences listed here. |
Composite parts
| Name | Type | Description | Length | Status |
|---|---|---|---|---|
| BBa_K4706015 | Composite | SrpR repressable PHO5 phosphate dependent cell death | 3532 | |
| BBa_K733012 | Composite | xylR+PxylA+RBS+ydcE+Ptms+RBS+ydcD: Cell Growth Inhibition Device | 2146 | In stock |
| BBa_K864150 | Composite | Cat-SacB cassette for scarless chromosomal knockout | 2214 | It's complicated |
| BBa_K1631025 | Device | Colicin-E3 cassette (Medium) | 2290 | It's complicated |
| BBa_K1631024 | Device | Colicin-E3 cassette (Strong) | 2290 | It's complicated |
| BBa_K4321007 | Composite | Cyt1Aa Cassette | 2180 | |
| BBa_K4321009 | Composite | Cyt2Ba Cassette | 1659 | |
| BBa_K112808 | Device | Enterobacteria phage T4 Lysis Device - no promoter | 1785 | It's complicated |
| BBa_K4706013 | Composite | Gal1 induced RFP A2 AtBAG6 | 2955 | |
| BBa_K2110008 | Regulatory | inclusion body induced cell lysis system | 665 | It's complicated |
| BBa_K515106 | Composite | J23103 promoer - RBS B0034-RFP E1010 - Holin K112805 - endolysin K112806 | 1973 | Not in stock |
| BBa_K3848004 | Composite | Lactate regulated kill switch | 2837 | |
| BBa_K137131 | Device | Lactose inducible lysis cassette expression | 2923 | It's complicated |
| BBa_K112020 | Device | Lambda phage lysis device | 2730 | It's complicated |
| BBa_K112019 | Device | Lambda phage lysis device | 2730 | It's complicated |
| BBa_K112018 | Device | Lambda phage lysis device | 2730 | It's complicated |
| BBa_K112023 | Device | Lambda phage lysis device - no promoter | 1499 | It's complicated |
| BBa_K112022 | Device | Lambda phage lysis device - no promoter | 1499 | It's complicated |
| BBa_K112021 | Device | Lambda phage lysis device - no promoter | 1499 | It's complicated |
| BBa_K358019 | Composite | Lysis cassette regulated by lacP | 1473 | It's complicated |
| BBa_K358023 | Composite | Lysisbox -type1- ver.2 | 2699 | Not in stock |
| BBa_K1631018 | Generator | Pconst(Strong) -rbs- CheZ -d.term | 823 | It's complicated |
| BBa_K1631012 | Generator | Plac -rbs- Barnase -rbs- GFP -d.term | 1330 | It's complicated |
| BBa_K1631023 | Generator | Plac -rbs- Barstar -d.term | 485 | It's complicated |
| BBa_K1631022 | Generator | Plux -rbs- Barnase -rbs- GFP -d.term | 1330 | It's complicated |
| BBa_K1054001 | Coding | protein E of Phage phiX174 under pBAD | 572 | It's complicated |
| BBa_K4706018 | Plasmid | pSB1C30YIpHR-HO Gal1 induced RFP A2 AtBAG6 | 6025 | |
| BBa_K4706019 | Plasmid | pSB1C30YIpHR-HO SrpR-PHO5 phosphate dependent cell death | 6602 | |
| BBa_K2150125 | Composite | Ptet + RBS + RiboJ + toxin 5980 CDS | 1221 | Not in stock |
| BBa_K748006 | Composite | S.aureus killing device. Truncated lysostaphin coding sequence with IPTG inducible promoter. | 959 | It's complicated |
| BBa_K772101 | Composite | Self-Destruction Kit: Endolysin + Holin | 839 | Not in stock |