Device

Part:BBa_K137131

Designed by: Josh Michener   Group: iGEM08_Caltech   (2008-10-23)

Lactose inducible lysis cassette expression

Lactose inducible expression of the phage lambda lysis cassette. Constitutive expression of lacI ensures tight repression of the promoter B4 except in the presence of lactose. Analogous to K137132 except with the lysis cassette replacing GFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1208
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/celldeath
//function/regulation/transcriptional
Parameters
None