Part:BBa_K1054001
protein E of Phage phiX174 under pBAD
Prontein E is a membrane protein that inhibits MraY, an enzyme required for murein synthesis, and promotes cell lysis in a manner analogous to cell wall synthesis inhibitors like penicillin. However, it will keep the periplasmic space of Gram-negative strain intact. In this part, protein E will be expressed when L-arabinose is available.
The growth curve of transformed E.coli cells treated with different concentrations of L-arabinose varying from 0.2% to 1%. MgSO4 has been reported to allow the expression of protein E but inhibit lysis. MgSO4 was added 30min before the induction according to previous works by others.
Left: SEM picture of protein E lysed E.coli.
Right: SEM picture of protein E lysed E.coli with MgSO4 protocol. The tunnel is relatively bigger than the left one.
References
[1] S. Resch et al., Aqueous release and purification of poly(b-hydroxybutyrate) from Escherichia coli, Journal of Biotechnology 65 (1998) 173–182
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| n/a | protein E of Phage phiX174 under pBAD |