Device

Part:BBa_K112020

Designed by: Bing Xia   Group: iGEM08_UC_Berkeley   (2008-10-24)

Lambda phage lysis device

Lysozyme and holin production are driven by pBad, and antiholin is driven by PconC6.

This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 33
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 33
    Illegal NheI site found at 1184
    Illegal NheI site found at 2230
    Illegal NheI site found at 2253
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal prefix found in sequence at 33
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 33
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 33
    Illegal NgoMIV site found at 2616
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1050


[edit]
Categories
//function/celldeath
Parameters
None