Composite

Part:BBa_K864150

Designed by: Erik Lundin   Group: iGEM12_Uppsala   (2012-09-25)

Cat-SacB cassette for scarless chromosomal knockout

Cat-SacB

Consists of chloramphenicol resistance marker BBa_P1004 and the Bacillus subtilis levansucrase SacB BBa_K322921

Usage and Biology

Cat-SacB.png

iGEM12_Uppsala_University: E. coli strain carrying a chromosomally integrated copy of cat-sacB (BBa_K864150) plated on a sucrose plate (A) and on a chloramphenicol plate (B). E. coli wildtype strain MG1655 was used as a control.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters

[edit]
Categories
//cds/selectionmarker
//chassis/prokaryote/ecoli
//chassis/prokaryote/salmonella
//function/celldeath
Parameters
None