Part:BBa_K4362023

42H-gal-dCas9

This is the plasmid that carried sequence of CBE, and also, there are some genes on it to make it feasible to be selected in Saccharomyces cerevisiae

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 4223
  Illegal EcoRI site found at 5902
  Illegal EcoRI site found at 6131
  Illegal EcoRI site found at 11134
  Illegal XbaI site found at 607
  Illegal PstI site found at 11044
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 4223
  Illegal EcoRI site found at 5902
  Illegal EcoRI site found at 6131
  Illegal EcoRI site found at 11134
  Illegal PstI site found at 11044
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 4223
  Illegal EcoRI site found at 5902
  Illegal EcoRI site found at 6131
  Illegal EcoRI site found at 11134
  Illegal BglII site found at 4508
  Illegal BglII site found at 4762
  Illegal BglII site found at 7366
  Illegal BamHI site found at 8169
  Illegal XhoI site found at 9199
  Illegal XhoI site found at 10350
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 4223
  Illegal EcoRI site found at 5902
  Illegal EcoRI site found at 6131
  Illegal EcoRI site found at 11134
  Illegal XbaI site found at 607
  Illegal PstI site found at 11044
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 4223
  Illegal EcoRI site found at 5902
  Illegal EcoRI site found at 6131
  Illegal EcoRI site found at 11134
  Illegal XbaI site found at 607
  Illegal PstI site found at 11044
  Illegal NgoMIV site found at 9741
  Illegal AgeI site found at 3826
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 2437
  Illegal BsaI site found at 4481
  Illegal SapI.rc site found at 3519
  Illegal SapI.rc site found at 4334
  Illegal SapI.rc site found at 7665