Part:BBa_K4362019

Micro Venus-hyg

This is the plasmid that carried Micro Verus's sequence, and also, there is a HphNT1 gene on it to select in Saccharomyces cerevisiae

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 774
  Illegal XbaI site found at 5980
  Illegal SpeI site found at 3041
  Illegal SpeI site found at 5315
  Illegal SpeI site found at 7785
  Illegal SpeI site found at 8292
  Illegal PstI site found at 864
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 774
  Illegal NheI site found at 4175
  Illegal NheI site found at 5073
  Illegal SpeI site found at 3041
  Illegal SpeI site found at 5315
  Illegal SpeI site found at 7785
  Illegal SpeI site found at 8292
  Illegal PstI site found at 864
  Illegal NotI site found at 2534
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 774
  Illegal BglII site found at 3028
  Illegal BglII site found at 5983
  Illegal BglII site found at 6078
  Illegal BamHI site found at 8282
  Illegal XhoI site found at 1558
  Illegal XhoI site found at 2510
  Illegal XhoI site found at 3220
  Illegal XhoI site found at 3990
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 774
  Illegal XbaI site found at 5980
  Illegal SpeI site found at 3041
  Illegal SpeI site found at 5315
  Illegal SpeI site found at 7785
  Illegal SpeI site found at 8292
  Illegal PstI site found at 864
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 774
  Illegal XbaI site found at 5980
  Illegal SpeI site found at 3041
  Illegal SpeI site found at 5315
  Illegal SpeI site found at 7785
  Illegal SpeI site found at 8292
  Illegal PstI site found at 864
  Illegal NgoMIV site found at 2167
  Illegal AgeI site found at 7178
  Illegal AgeI site found at 7565
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 8234
  Illegal BsaI.rc site found at 9670
  Illegal SapI site found at 8587
  Illegal SapI site found at 10691