Difference between revisions of "Plasmid backbones/Other standards"

Revision as of 21:44, 4 September 2008

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Or get some help on plasmid backbones.

Partial BioBrick cloning site

• Part:BBa_J61031 - partial BioBrick cloning site, amplifiable BAC plasmid
• Part:BBa_I714945 - partial BioBrick cloning site
• Part:BBa_I713002 - no features entered

BioBrick-compatible plasmid

• Part:BBa_J23006 - BioBrick-compatible plasmid for cloning parts downstream of R0040 promoter, needs to be reentered SpeI->EcoRI?
• Part:BBa_J61002 - BioBrick-compatible plasmid for cloning protein generators downstream of R0040
• Part:BBa_J61003 - BioBrick-compatible plasmid for cloning ORFs downstream of R0040.B0034 or assembling promoter/RBS's in front of GFP.B0015
• Part:BBa_J23018 - BioBrick-compatible plasmid for cloning ORFs downstream of R0040.B0034 or assembling promoter/RBS's in front of RFP.B0015
• Part:BBa_J23019 - BioBrick-compatible plasmid for cloning ORFs downstream of R0040.B0034 or assembling promoter/RBS's in front of RFP.B0015
• Part:BBa_J61035 - BioBrick-compatible plasmid with GenR between E and X
• Part:BBa_I732950 - promoter entered between EcoRI and XbaI, no features entered
• Part:BBa_I732951 - promoter entered between EcoRI and XbaI, no features entered

Different assembly standard

• Part:BBa_I716101 - not a BioBrick plasmid ... berkeley standard

Too many BioBrick sites

• Part:BBa_I713001 - no features entered
• Part:BBa_I714945 - multiple BioBrick enzyme sites

Not a BioBrick plasmid at all

• Part:BBa_J61007
• Part:BBa_J61009
• Part:BBa_I713003
• Part:BBa_I751000 - single EcoRI site

Actually a part in a plasmid

• Part:BBa_I713003 - not a BioBrick plasmid