Plasmid backbones/Synthesis
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Plasmids used in DNA synthesis of BioBrick parts
Most DNA synthesis companies do not currently use BioBrick plasmid backbones to clone synthesized BioBrick parts. Therefore, some parts in the Registry are available in non-standard plasmid backbones because they were constructed via direct DNA synthesis. Here is a list of plasmid backbones used by assorted DNA synthesis companies.
| Name | Description | Resistance | Replicon | Copy number | Chassis | Length |
|---|---|---|---|---|---|---|
| BBa_J70003 | Geneart Cloning Plasmid pGA4 | A | 2961 | |||
| BBa_J70004 | BioBasic Cloning Plasmid pUC57 | A | 2710 | |||
| BBa_J70022 | GENEART pGA4 plasmid | 2978 | ||||
| BBa_K2812001 | Coding sequence for trunctated Lysostaphin | 738 | ||||
| BBa_K2812003 | Coding sequence for trunctated Lysostaphin regulated by T7-promoter | 776 | ||||
| BBa_K2812004 | Coding sequence for trunctated Lysostaphin fused to His-tagged HlyA | 1458 | ||||
| BBa_K2812005 | Coding sequence for trunctated Lysostaphin with HlyA and His6-tag regulated by T7-promoter | 1496 | ||||
| BBa_K2812006 | Coding sequence for Pyocin S5 with HlyA and His6-tag | 2217 | ||||
| BBa_K2812007 | Coding sequence for Pyocin S5 with HlyA and His6-tag regulated by pBAD-ara promoter | 2288 | ||||
| pSB1A1 | pUC19 with a BioBrick cloning site (Replaced by pSB1A3) | A | pMB1 | 500-700 | 2708 |