Part:BBa_J61035

EcoRI-GenR-XbaI

Selectable marker inserted between EcoRI and XbaI restriction sites. The sequence inluded describes plasmid pSB1A2-b0034 (ribosome binding site part) with this cassette inserted between EcoRI and XbaI. This part facilitates the transfer of small parts during biobrick assembly. Because it is fairly large in size, EcoRI/SpeI digests of the plasmid can be readily gel purified. Because it contains a selectable marker, cloning products containing the cassette can be selected on gentamicin plates. Note that the R6K origin of replication is also present within this cassette. It will be silent in any non-pir strain (MG1655, DH10B, DH5a, MC1061, MC4100, HB101, etc.)

• RS 19:58, 24 November 2008 (UTC): The original entry of this part included both a plasmid and plasmid backbone. It has been split into a plasmid backbone and part. The physical Registry distribution has been updated accordingly.

The original part is now BBa_J61035-B0034.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal suffix found in sequence at 1
  Illegal EcoRI site found at 2058
  Illegal XbaI site found at 3533
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 2058
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal NotI site found at 9
  Illegal NotI site found at 2514
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 2058
  Illegal BglII site found at 2750
  Illegal BglII site found at 3487
  Illegal XhoI site found at 3354
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal suffix found in sequence at 2
  Illegal EcoRI site found at 2058
  Illegal XbaI site found at 3533
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 2058
  Illegal XbaI site found at 3533
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1097