Difference between revisions of "Plasmid backbones/Other standards"
| Line 25: | Line 25: | ||
==Tumor killing bacteria testbed== | ==Tumor killing bacteria testbed== | ||
| + | |||
| + | Chris Anderson has submitted a set of plasmids associated with his paper on ''Environmentally Controlled Invasion of Cancer Cells by Engineered Bacteria''. Please read the paper for details. | ||
<parttable>tumor_killing_bacteria_plasmid</parttable> | <parttable>tumor_killing_bacteria_plasmid</parttable> | ||
| + | <biblio> | ||
| + | #Anderson pmid=16330045 | ||
| + | </biblio> | ||
==Other miscellaneous vectors== | ==Other miscellaneous vectors== | ||
The Registry also have miscellaneous other plasmids and plasmids backbones. These plasmids and plasmid backbones have not undergone Registry curation, but we include them here for completeness. | The Registry also have miscellaneous other plasmids and plasmids backbones. These plasmids and plasmid backbones have not undergone Registry curation, but we include them here for completeness. | ||
Revision as of 18:42, 28 September 2008
| Part assembly | System operation | Protein expression | Assembly of protein fusions | Part measurement | Screening of part libraries | Building BioBrick vectors | DNA synthesis | Other standards | Archive |
| Or get some help on plasmid backbones. |
Plasmids used in DNA synthesis of BioBrick® parts
Most DNA synthesis companies do not currently use BioBrick® plasmid backbones to clone synthesized BioBrick® parts. Therefore, some parts in the Registry are available in non-standard plasmid backbones because they were constructed via direct DNA synthesis. Here is a list of plasmid backbones used by assorted DNA synthesis companies.
| Name | Description | Resistance | Replicon | Copy number | Chassis | Length |
|---|---|---|---|---|---|---|
| BBa_J70003 | Geneart Cloning Plasmid pGA4 | A | 2961 | |||
| BBa_J70004 | BioBasic Cloning Plasmid pUC57 | A | 2710 | |||
| BBa_J70022 | GENEART pGA4 plasmid | 2978 | ||||
| BBa_K2812001 | Coding sequence for trunctated Lysostaphin | 738 | ||||
| BBa_K2812003 | Coding sequence for trunctated Lysostaphin regulated by T7-promoter | 776 | ||||
| BBa_K2812004 | Coding sequence for trunctated Lysostaphin fused to His-tagged HlyA | 1458 | ||||
| BBa_K2812005 | Coding sequence for trunctated Lysostaphin with HlyA and His6-tag regulated by T7-promoter | 1496 | ||||
| BBa_K2812006 | Coding sequence for Pyocin S5 with HlyA and His6-tag | 2217 | ||||
| BBa_K2812007 | Coding sequence for Pyocin S5 with HlyA and His6-tag regulated by pBAD-ara promoter | 2288 | ||||
| BBa_K5293014 | pHREAC_eGFP_ER | 10477 | ||||
| pSB1A1 | pUC19 with a BioBrick cloning site (Replaced by pSB1A3) | A | pMB1 | 500-700 | 2708 |
Berkeley assembly standard plasmid backbones
Researchers at UC Berkeley have developed a new assembly standard. See [http://openwetware.org/wiki/The_BioBricks_Foundation:Standards/Technical/Formats#The_Berkeley_.28BBb.29_Format the BioBricks Foundation wiki] for more details.
There are no parts for this table
Tumor killing bacteria testbed
Chris Anderson has submitted a set of plasmids associated with his paper on Environmentally Controlled Invasion of Cancer Cells by Engineered Bacteria. Please read the paper for details.
| Name | Description | Resistance | Copy Number | Origin | Length | Default Insert | PCR |
|---|---|---|---|---|---|---|---|
| BBa_J61004 | pAC-TetInv | 5559 | |||||
| BBa_J61005 | pAC-LuxInv | 7181 | |||||
| BBa_J61006 | pBACr-AraInv | 10830 | |||||
| BBa_J61008 | pBACr-FdhInv | 9764 | |||||
| BBa_J61009 | pAC-LuxGFP | C | p15A | 5130 | p15A | p15A |
<biblio>
- Anderson pmid=16330045
</biblio>
Other miscellaneous vectors
The Registry also have miscellaneous other plasmids and plasmids backbones. These plasmids and plasmid backbones have not undergone Registry curation, but we include them here for completeness.