Plasmid
pAC-LuxGFP

Part:BBa_J61009

Designed by: John Anderson   Group: Arkin Lab   (2006-08-04)

pAC-LuxGFP

JCAJ61009map.png JCAJ61009data.jpg

pAC-LuxGFP places GFP under the wildtype Vibrio lux device. It's behavior is described in the cytometry data to the right.

This data, and more on this construct are described in:

<biblio>

  1. Anderson pmid=16330045

</biblio>

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1733
    Illegal EcoRI site found at 2688
    Illegal XbaI site found at 588
    Illegal XbaI site found at 5084
    Illegal PstI site found at 138
    Illegal PstI site found at 2498
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1733
    Illegal EcoRI site found at 2688
    Illegal NheI site found at 2657
    Illegal NheI site found at 4242
    Illegal PstI site found at 138
    Illegal PstI site found at 2498
    Illegal NotI site found at 6
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1733
    Illegal EcoRI site found at 2688
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1733
    Illegal EcoRI site found at 2688
    Illegal XbaI site found at 588
    Illegal XbaI site found at 5084
    Illegal PstI site found at 138
    Illegal PstI site found at 2498
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1733
    Illegal EcoRI site found at 2688
    Illegal XbaI site found at 588
    Illegal XbaI site found at 5084
    Illegal PstI site found at 138
    Illegal PstI site found at 2498
    Illegal AgeI site found at 4328
    Illegal AgeI site found at 4652
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2781
    Illegal BsaI site found at 2959
    Illegal BsaI.rc site found at 2423


[edit]
Categories
//function/tumorkillingbacteria
//plasmid
Parameters
chassis
copies
mcsNonstandard
originp15A
resistanceC