Difference between revisions of "Plasmid backbones/Other standards"
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==Tumor killing bacteria testbed== | ==Tumor killing bacteria testbed== | ||
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Revision as of 22:20, 23 September 2008
| Part assembly | System operation | Protein expression | Assembly of protein fusions | Part measurement | Screening of part libraries | Building BioBrick vectors | DNA synthesis | Other standards | Archive |
| Or get some help on plasmid backbones. |
Plasmids used in DNA synthesis of BioBrick® parts
Most DNA synthesis companies do not currently use BioBrick® plasmid backbones to clone synthesized BioBrick® parts. Therefore, some parts in the Registry are available in non-standard plasmid backbones because they were constructed via direct DNA synthesis. Here is a list of plasmid backbones used by assorted DNA synthesis companies.
| Name | Description | Resistance | Replicon | Copy number | Chassis | Length |
|---|---|---|---|---|---|---|
| BBa_J70003 | Geneart Cloning Plasmid pGA4 | A | 2961 | |||
| BBa_J70004 | BioBasic Cloning Plasmid pUC57 | A | 2710 | |||
| BBa_J70022 | GENEART pGA4 plasmid | 2978 | ||||
| BBa_K2812001 | Coding sequence for trunctated Lysostaphin | 738 | ||||
| BBa_K2812003 | Coding sequence for trunctated Lysostaphin regulated by T7-promoter | 776 | ||||
| BBa_K2812004 | Coding sequence for trunctated Lysostaphin fused to His-tagged HlyA | 1458 | ||||
| BBa_K2812005 | Coding sequence for trunctated Lysostaphin with HlyA and His6-tag regulated by T7-promoter | 1496 | ||||
| BBa_K2812006 | Coding sequence for Pyocin S5 with HlyA and His6-tag | 2217 | ||||
| BBa_K2812007 | Coding sequence for Pyocin S5 with HlyA and His6-tag regulated by pBAD-ara promoter | 2288 | ||||
| BBa_K5293014 | pHREAC_eGFP_ER | 10477 | ||||
| pSB1A1 | pUC19 with a BioBrick cloning site (Replaced by pSB1A3) | A | pMB1 | 500-700 | 2708 |
Berkeley assembly standard plasmid backbones
Researchers at UC Berkeley have developed a new assembly standard. See [http://openwetware.org/wiki/The_BioBricks_Foundation:Standards/Technical/Formats#The_Berkeley_.28BBb.29_Format the BioBricks Foundation wiki] for more details.
There are no parts for this table
Tumor killing bacteria testbed
| Name | Description | Resistance | Copy Number | Origin | Length | Default Insert | PCR |
|---|---|---|---|---|---|---|---|
| BBa_J61004 | pAC-TetInv | 5559 | |||||
| BBa_J61005 | pAC-LuxInv | 7181 | |||||
| BBa_J61006 | pBACr-AraInv | 10830 | |||||
| BBa_J61008 | pBACr-FdhInv | 9764 | |||||
| BBa_J61009 | pAC-LuxGFP | C | p15A | 5130 | p15A | p15A |
- Part:BBa_J61004
- Part:BBa_J61005
- Part:BBa_J61006
- Part:BBa_J61007 - is this part of TKD?
- Part:BBa_J61008
- Part:BBa_J61009
Other miscellaneous vectors
The Registry also have miscellaneous other plasmids and plasmids backbones. These plasmids and plasmid backbones have not undergone Registry curation, but we include them here for completeness.