Difference between revisions of "Plasmid backbones/Other standards"
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| + | ==Plasmids used in DNA synthesis of BioBrick® parts== | ||
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| + | Most DNA synthesis companies do not currently use BioBrick® plasmids to clone synthesized BioBrick® parts. Therefore, some parts in the Registry are available in non-standard plasmids because they were constructed via direct DNA synthesis. Here is a list of those plasmids. | ||
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| + | <parttable>synthesis_plasmid</parttable> | ||
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==Berkeley assembly standard plasmid backbones== | ==Berkeley assembly standard plasmid backbones== | ||
<parttable>berkeley_plasmid</parttable> | <parttable>berkeley_plasmid</parttable> | ||
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==Tumor killing bacteria testbed== | ==Tumor killing bacteria testbed== | ||
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==Other useful vectors== | ==Other useful vectors== | ||
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*[[Part:BBa_I751000]] - useful for amplifying pBR322 origin | *[[Part:BBa_I751000]] - useful for amplifying pBR322 origin | ||
==Other random vectors== | ==Other random vectors== | ||
Revision as of 18:50, 19 September 2008
| Part assembly | System operation | Protein expression | Assembly of protein fusions | Part measurement | Screening of part libraries | Building BioBrick vectors | DNA synthesis | Other standards | Archive |
| Or get some help on plasmid backbones. |
Plasmids used in DNA synthesis of BioBrick® parts
Most DNA synthesis companies do not currently use BioBrick® plasmids to clone synthesized BioBrick® parts. Therefore, some parts in the Registry are available in non-standard plasmids because they were constructed via direct DNA synthesis. Here is a list of those plasmids.
| Name | Description | Resistance | Replicon | Copy number | Chassis | Length |
|---|---|---|---|---|---|---|
| BBa_J70003 | Geneart Cloning Plasmid pGA4 | A | 2961 | |||
| BBa_J70004 | BioBasic Cloning Plasmid pUC57 | A | 2710 | |||
| BBa_J70022 | GENEART pGA4 plasmid | 2978 | ||||
| BBa_K2812001 | Coding sequence for trunctated Lysostaphin | 738 | ||||
| BBa_K2812003 | Coding sequence for trunctated Lysostaphin regulated by T7-promoter | 776 | ||||
| BBa_K2812004 | Coding sequence for trunctated Lysostaphin fused to His-tagged HlyA | 1458 | ||||
| BBa_K2812005 | Coding sequence for trunctated Lysostaphin with HlyA and His6-tag regulated by T7-promoter | 1496 | ||||
| BBa_K2812006 | Coding sequence for Pyocin S5 with HlyA and His6-tag | 2217 | ||||
| BBa_K2812007 | Coding sequence for Pyocin S5 with HlyA and His6-tag regulated by pBAD-ara promoter | 2288 | ||||
| BBa_K5293014 | pHREAC_eGFP_ER | 10477 | ||||
| pSB1A1 | pUC19 with a BioBrick cloning site (Replaced by pSB1A3) | A | pMB1 | 500-700 | 2708 |
Berkeley assembly standard plasmid backbones
There are no parts for this table
Tumor killing bacteria testbed
- Part:BBa_J61004
- Part:BBa_J61005
- Part:BBa_J61006
- Part:BBa_J61007 - is this part of TKD?
- Part:BBa_J61008
- Part:BBa_J61009
Other useful vectors
- Part:BBa_I751000 - useful for amplifying pBR322 origin