Difference between revisions of "Plasmid backbones/Other standards"

Revision as of 19:12, 19 September 2008


Part assembly	System operation	Protein expression	Assembly of protein fusions	Part measurement	Screening of part libraries	Building BioBrick vectors	DNA synthesis	Other standards	Archive

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Plasmids used in DNA synthesis of BioBrick® parts

Most DNA synthesis companies do not currently use BioBrick® plasmids to clone synthesized BioBrick® parts. Therefore, some parts in the Registry are available in non-standard plasmids because they were constructed via direct DNA synthesis. Here is a list of those plasmids.

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Name	Description	Resistance	Replicon	Copy number	Length
BBa_J70003	Geneart Cloning Plasmid pGA4	A			2961
BBa_J70004	BioBasic Cloning Plasmid pUC57	A			2710
BBa_J70022	GENEART pGA4 plasmid				2978
BBa_K2812001	Coding sequence for trunctated Lysostaphin				738
BBa_K2812003	Coding sequence for trunctated Lysostaphin regulated by T7-promoter				776
BBa_K2812004	Coding sequence for trunctated Lysostaphin fused to His-tagged HlyA				1458
BBa_K2812005	Coding sequence for trunctated Lysostaphin with HlyA and His6-tag regulated by T7-promoter				1496
BBa_K2812006	Coding sequence for Pyocin S5 with HlyA and His6-tag				2217
BBa_K2812007	Coding sequence for Pyocin S5 with HlyA and His6-tag regulated by pBAD-ara promoter				2288
BBa_K5293014	pHREAC_eGFP_ER				10477
pSB1A1	pUC19 with a BioBrick cloning site (Replaced by pSB1A3)	A	pMB1	500-700	2708

Berkeley assembly standard plasmid backbones

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Tumor killing bacteria testbed