Part:BBa_K538130

pBAD + RBS

K538130

This part was submitted by team [http://2011.igem.org/Team:Amsterdam Amsterdam 2011] as part of their project: icE. coli. It was used as a construction intermediate, to create the following composite parts:

• BBa_K538300
• BBa_K538301
• BBa_K538302
• BBa_K538303
• BBa_K538304
• BBa_K538310
• BBa_K538311

Note this part contains no Bricks that are new to the registry; its subparts, BBa_K206000 and BBa_B0034, were part of 2011's Spring Distribution kit. Team [http://2011.igem.org/Team:Amsterdam Amsterdam 2011] used it to create the various protein generators listed above, by appending one of the parts below:

• BBa_K538000: Cpn10 (from Oleispira antarctica)
• BBa_K538001: Cpn60 (from Oleispira antarctica)
• BBa_K538002: SheDnak (from Shewanella sp. AC10)
• BBa_K538003: CspA (from Polaribacter irgensii)
• BBa_K538004: CspC (from Polaribacter irgensii)

In addition to this, for the creation of BBa_K538310 and BBa_K538211, one of these parts was also appended:

• BBa_K538100: RBS + Cpn10
• BBa_K538101: RBS + Cpn60

These bricks all encode a protein that should confer a degree of cold resistance when expressed by E. coli. The biology behind these cold resistance BioBricks, or CryoBricks, is explained in greater detail on the pages of the individual coding regions.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 125
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 65
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]