Part:BBa_K538302

pBAD + RBS + SheDnaK

pBAD:SDnaK K538302

This part was submitted by team [http://2011.igem.org/Team:Amsterdam Amsterdam 2011] as part of their project: icE. coli. Its purpose is to express the DnaK protein from Shewanella sp. AC10 (BBa_K538002) under control of the strong arabinose-induced promoter team [http://2009.igem.org/Team:British_Columbia British Columbia 2009] derived from pBAD (BBa_K206000). Heterologous expression of this protein has rescued E. coli DnaK-null mutants, and facilitated their growth at 15°C. This Brick's designers intend to use it to culture 'normal' E. coli faster at temperatures below its optimum (37°C), possibly even facilitating growth below the minimal growth temperature of 7°C.

Refer to the main page of the coding region (SheDnaK: BBa_K538002) for more information on how SheDnaK facilitates cold resistance in E. coli.

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 125
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 903
  Illegal BamHI site found at 65
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]