Difference between revisions of "Test2"

(Fluorescent Proteins)
(Fluorescent Proteins)
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|Reference
 
|Reference
 
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|Cerulean
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|style="background:#FFFF00" |Cerulean
 
|433
 
|433
 
|475
 
|475
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|GFPmut3b
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|style="background:#FFFF00" |GFPmut3b
 
|501
 
|501
 
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|511
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|amilGFP
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|style="background:#FFFF00" |amilGFP
 
|503
 
|503
 
|512
 
|512
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|[http://www.ncbi.nlm.nih.gov/nuccore/ay646067 GeneBank]
 
|[http://www.ncbi.nlm.nih.gov/nuccore/ay646067 GeneBank]
 
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|mVenus
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|style="background:#FFFF00" |mVenus
 
|515
 
|515
 
|528
 
|528
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|mOrange
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|style="background:#FFFF00" |mOrange
 
|548
 
|548
 
|562
 
|562
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|mStrawbery
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|style="background:#FFFF00" |mStrawbery
 
|574
 
|574
 
|596
 
|596
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|mRFP1
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|style="background:#FFFF00" |mRFP1
 
|584
 
|584
 
|607
 
|607
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|mCherry
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|style="background:#FFFF00" |mCherry
 
|587
 
|587
 
|610
 
|610
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|eforRed
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|style="background:#FFFF00" |eforRed
 
|589
 
|589
 
|609
 
|609
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|[http://www.ncbi.nlm.nih.gov/nuccore/EU498726 GeneBank]
 
|[http://www.ncbi.nlm.nih.gov/nuccore/EU498726 GeneBank]
 
|-
 
|-
|cjBlue
+
|style="background:#FFFF00" |cjBlue
 
|610
 
|610
 
|
 
|
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|[http://www.ncbi.nlm.nih.gov/pubmed/17028187 PubMed]
 
|[http://www.ncbi.nlm.nih.gov/pubmed/17028187 PubMed]
 
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|aeBlue
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|style="background:#FFFF00" |aeBlue
 
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|
 
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|[http://www.ncbi.nlm.nih.gov/pubmed/16164420 PubMed]
 
|[http://www.ncbi.nlm.nih.gov/pubmed/16164420 PubMed]
 
|-
 
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|amilCP
+
|style="background:#FFFF00" |amilCP
 
|588
 
|588
 
|NA
 
|NA

Revision as of 21:35, 6 March 2013

Icon Sensors.png


Biosafety: Parts improving biological safety, containment.
Biosynthesis: Parts involved in the production of chemicals and metabolites are listed here.
Biodegradation: Parts involved in the degradation of chemicals are listed here.
Cell-cell signaling and quorum sensing: Parts involved in intercellular signaling and quorum sensing between bacteria.
Cell death: Parts involved in killing cells.
Coliroid: Parts involved in taking a bacterial photograph.
Conjugation: Parts involved in DNA conjugation between bacteria.
DNA binding: Parts involved in xxx.
DNA recombination: Parts involved in DNA recombination are listet here.
Ions: Parts involved in sensing, transport and binding of different ions are listet in here.
Ligand Binding: Parts involved in binding chemical structures are listet here.
Light production and sensing: Parts involved in generation and sensing of light are listet here.
Motility and chemotaxis: Parts involved in motility or chemotaxis of cells are listet here.
Odor production and sensing: Parts that produce or sense odorants aer listet here.
Sensors: Parts used as biosensors are listed here.
Viral vectors: Parts involved in the production and modification of Viral vectors are listet here..












Fluorescent Proteins

Featured Parts, [http://www.microscopyu.com/articles/livecellimaging/fpintro.html Nikkon], [http://2012.igem.org/Team:Uppsala_University/Chromoproteins Chromoproteins Uppsala] https://parts.igem.org/Part:BBa_K180009 mBanana

Name Exitation [nm] Emmission [nm] Quant. yield Molar extinct. BioBrick RFC10 BioBrick RFC25 Reference
Cerulean 433 475 0.62 43000 BBa_E2020
GFPmut3b 501 511 BBa_E0040
amilGFP 503 512 0.67 75200 BBa_K592010 [http://www.ncbi.nlm.nih.gov/nuccore/ay646067 GeneBank]
mVenus 515 528 0.57 92200 BBa_I757008
mOrange 548 562 0.69 71000 BBa_E2050
mStrawbery 574 596 0.29 90000 BBa_K399001
mRFP1 584 607 0.25 50000 BBa_E1010
mCherry 587 610 0.22 72000 BBa_E2060
eforRed 589 609 0.16 111300 BBa_K592012 [http://www.ncbi.nlm.nih.gov/nuccore/EU498726 GeneBank]
cjBlue 610 BBa_K592011 [http://www.ncbi.nlm.nih.gov/pubmed/17028187 PubMed]
aeBlue BBa_K864401 [http://www.ncbi.nlm.nih.gov/pubmed/16164420 PubMed]
amilCP 588 NA NA 8760 BBa_K592009 [http://www.ncbi.nlm.nih.gov/nuccore/AY646075 GeneBank]



Name mVenus (YFP) mCherry (CFP) mOrange mCerulean RFP eforRed cjBlue aeBlue amilGFP amilCP mStrawberry
Color yellow light pink
BioBricks RFC10 BBa_E0040 ?? E1010 BBa_E2060 BBa_J06504 BBa_E2050 BBa_E2020 BBa_K592012 BBa_K592011 BBa_K864401 BBa_K592010 BBa_K592009 BBa_K399001
BioBricks RFC25 BBa_I757008

Fluciferases

Name Photinus luciferase Renilla luciferase Gaussia luciferase Nano Luciferase
Reference [http://www.reportergene.com/2013/01/the-nanoluc-luciferase-reporter-dossier.html]
Organism
Substrate
pH optimum
Substrate
Amino acids
Molecular mass
BioBricks RFC 10
BioBricks RFC 25 BBa_I712019 BBa_J52008 BBa_K509005

=Photoproteins= (Aequorin, Obelin