Difference between revisions of "Plasmid backbones/Measurement"

Revision as of 20:34, 17 September 2008


Part assembly	System operation	Protein expression	Assembly of protein fusions	Part measurement	Screening of part libraries	Building BioBrick vectors	DNA synthesis	Other standards	Archive

Or get some help on plasmid backbones.

Some plasmids are designed to facilitate the measurement of different part classes:

Part:BBa_J23101 - A collection of promoters characterized by Chris Anderson. The plasmid backbone from these parts can be used to measure the activity of other promoters based on expression of RFP.
There is a kit of components available that is described here to measure promoter activity in standard units. The kit makes use of pSB3K3 as the plasmid and BBa_E0240 (GFP) to measure promoter activity.

Part:pSB1A10 - This dual reporter (GFP and RFP) plasmid can be used to measure the transfer curve of inverter devices.

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 ===Promoters===
 *[[Part:BBa_J23101]] - A collection of promoters characterized by Chris Anderson.  The plasmid backbone from these parts can be used to measure the activity of other promoters based on expression of RFP.
-*There is a kit of components available that is described [[Measurement|here]] to measure promoter activity in standard units.  The kit makes use of [[Part:pSB3K3]] as the plasmid and [[Part:BBa_E0240]] (GFP) to measure promoter activity.
+*There is a kit of components available that is described [[Measurement|here]] to measure promoter activity in standard units.  The kit makes use of [[Part:pSB3K3|pSB3K3]] as the plasmid and [[Part:BBa_E0240|BBa_E0240]] (GFP) to measure promoter activity.
 ===Inverters===
 *[[Part:pSB1A10]] - This dual reporter (GFP and RFP) plasmid can be used to measure the transfer curve of [https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=inverter inverter devices].