Difference between revisions of "Help:2016 DNA Distribution"

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[[Category:Distribution Kit]]
 
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Whether you’re new to iGEM and the Registry of Standard Biological Parts or an experienced participant, please make sure to read through the Distribution Handbook.
+
Whether you’re new to iGEM and the Registry of Standard Biological Parts or an experienced participant, make sure to read through the Distribution Handbook.
 
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−
<p style="text-indent: 20px;">The iGEM 2016 DNA Distribution contains over 1000 high-quality part samples as dried (miniprepped) DNA. Each sample is [[Help:Quality_Control|QC tested]] through sequencing and AB test plates.</p>  
+
<p style="text-indent: 20px;">The iGEM 2016 DNA Distribution contains over 1200 high-quality part samples as dried (miniprepped plasmid) DNA. Each sample is [[Help:Quality_Control|QC tested]] through sequencing and AB test plates.</p>  
  
−
<p>While there is not enough DNA for assembly right out of the kit, you will be able to [[#DNA Kit Plate Instructions|transform the DNA]] into competent cells and then make your own glycerol stocks of any part you wish.</p>
+
<p>While there is not enough DNA for [[Help:Assembly/3A_Assembly|3A Assembly]] right out of the kit, you can [[#DNA Kit Plate Instructions|transform a part]] into competent cells and make your own glycerol stock for future use.</p>
  
−
<p style="text-indent: 20px;">If you're new to the Registry, iGEM, or synthetic biology, you'll want to read through our [[Help:Learn|Help pages]] before you get started.</p>
+
<p style="text-indent: 20px;">When you receive your Distribution Kit, let us know through twitter ('''[https://twitter.com/hashtag/WelcomeToiGEM #WelcomeToiGEM]'''). If you're new to the Registry, iGEM, or synthetic biology, you will also want to read through our [[Help:Contents|Help pages]] before you get started.</p>
  
 
<center>Otherwise, <b>read on!</b></center>
 
<center>Otherwise, <b>read on!</b></center>
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<div class="redbox">Quality</div> In making this distribution, we selected only parts with samples that were sequence confirmed or ends confirmed (long parts). [[#DNA Distribution Kit Plates | More...]]<br /><br />
 
<div class="redbox">Quality</div> In making this distribution, we selected only parts with samples that were sequence confirmed or ends confirmed (long parts). [[#DNA Distribution Kit Plates | More...]]<br /><br />
  
−
<div class="redbox">Standards</div> All parts in Kit Plates 1 - 3, and 5 are in pSB1C3, the standard shipping plasmid backbone for the iGEM Registry. [[#Kit Plates 1 - 3 | More...]]<br /><br />
+
<div class="redbox">Standards</div> All parts in Kit Plates 1 - 3, and 5 - 6 are in pSB1C3, the standard shipping plasmid backbone for the iGEM Registry. [[#Kit Plates 1 - 3 | More...]]<br /><br />
  
−
<div class="redbox">Confidence</div> We’ve included the Transformation Efficiency Kit, so that you can test the transformation efficiency of your competent cells, before you start using the Distribution Kit Plates. [[#Transformation_Efficiency_Kit | More...]]<br /><br />
+
<div class="redbox">Confidence</div> We’ve included the Competent Cell Test Kit, so that you can test the transformation efficiency of your competent cells, before you start using the Distribution Kit Plates. [[#Competent_Cell_Test_Kit | More...]]<br /><br />
  
 
<div class="redbox">Inspected</div> All of the Distribution Kit Plates are visually inspected to make sure they contain the DNA needed for transformation.<br/>  
 
<div class="redbox">Inspected</div> All of the Distribution Kit Plates are visually inspected to make sure they contain the DNA needed for transformation.<br/>  
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==What's included in the 2016 Distribution==
 
==What's included in the 2016 Distribution==
−
<html><img src="https://static.igem.org/mediawiki/parts/0/08/2015_DNA_Distribution.jpg" style="float: left; width: 400px; padding: 0px 20px;"></html>
+
<html><img src="https://static.igem.org/mediawiki/parts/e/e9/2016_Distribution_Contents.jpg" style="float: left; width: 400px; padding: 0px 20px;"></html>
  
 
<b>Your 2016 DNA Distribution contains the following:</b><br />
 
<b>Your 2016 DNA Distribution contains the following:</b><br />
−
[[Image:Green_BulletPt.png|link=]] [[#DNA Distribution Kit Plates | DNA Distribution Kit Plates 1 - 5]]<br />
+
[[Image:Green_BulletPt.png|link=]] [[#DNA Distribution Kit Plates | DNA Distribution Kit Plates 1 - 6]]<br />
 
[[Image:Green_BulletPt.png|link=]] [[#Linearized Plasmid Backbones | Linearized Plasmid Backbones]]<br />
 
[[Image:Green_BulletPt.png|link=]] [[#Linearized Plasmid Backbones | Linearized Plasmid Backbones]]<br />
−
[[Image:Green_BulletPt.png|link=]] [[#Transformation_Efficiency_Kit | Transformation Efficiency Kit]]<br />
+
[[Image:Green_BulletPt.png|link=]] [[#Competent_Cell_Test_Kit | Competent Cell Test Kit]]<br />
−
[[Image:Green_BulletPt.png|link=]] [[#2015_Submission_Kit | 2015 Part Submission Kit]]<span style="color:red;"> NEW!</span><br />
+
[[Image:Green_BulletPt.png|link=]] [[#InterLab_Measurement_Kit | InterLab Measurement Kit]]<span style="color:red;"> NEW!</span><br />
−
[[Image:Green_BulletPt.png|link=]] iGEM Stickers and Pins!  
+
[[Image:Green_BulletPt.png|link=]] [[#2016_Submission_Kit | 2016 Part Submission Kit]]<span style="color:red;"> NEW!</span><br />
 +
[[Image:Green_BulletPt.png|link=]] iGEM Stickers and Pins! <br />
 +
[[Image:Green_BulletPt.png|link=]] Sponsor Material from PLOS and IDT <span style="color:red;"> NEW!</span>
  
−
If there is an issue with your distribution kit, please send us an email to ''hq (at) igem . org''
+
If there is an issue with your distribution kit, please send an email to ''hq (at) igem . org''
 
<div style="clear: both;"></div>
 
<div style="clear: both;"></div>
  
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====Kit Plates 1 - 3====
 
====Kit Plates 1 - 3====
  
−
<html><img src="https://static.igem.org/mediawiki/parts/7/70/2015_KP1-3.jpg" style="float: left; width: 300px; padding: 5px 20px 20px 20px;" /></html>
+
<html><img src="https://static.igem.org/mediawiki/parts/4/41/2016_Distribution_KP1-3.jpg" style="float: left; width: 300px; padding: 5px 20px 20px 20px;" /></html>
 
<div class="redbox">Standards</div> In addition to the new parts that have been submitted to the Registry, we have transferred over many of our existing sequence verified parts into pSB1C3, the [https://parts.igem.org/Help:Shipping Registry’s standard shipping plasmid backbone]. All parts in DNA Distribution Kit Plates 1 to 3 are in pSB1C3.  
 
<div class="redbox">Standards</div> In addition to the new parts that have been submitted to the Registry, we have transferred over many of our existing sequence verified parts into pSB1C3, the [https://parts.igem.org/Help:Shipping Registry’s standard shipping plasmid backbone]. All parts in DNA Distribution Kit Plates 1 to 3 are in pSB1C3.  
  
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====Kit Plate 4====
 
====Kit Plate 4====
  
−
<html><img src="https://static.igem.org/mediawiki/parts/0/0b/2015_KP4.jpg" style="float: left; width: 300px; padding: 5px 20px 20px 20px;" /></html>
+
<html><img src="https://static.igem.org/mediawiki/parts/8/8c/2016_Distribution_KP4.jpg" style="float: left; width: 300px; padding: 5px 20px 20px 20px;" /></html>
−
DNA Distribution Kit Plate 4 contains parts in a variety of Registry standard plasmid backbones (pSB1C3, pSB1A3, pSB3K3, etc).  
+
[https://parts.igem.org/assembly/plates.cgi?id=4398 DNA Distribution Kit Plate 4] contains parts in a variety of Registry standard plasmid backbones (pSB1C3, pSB1A3, pSB3K3, etc).  
 
'''On this plate you'll find...'''
 
'''On this plate you'll find...'''
 
*Registry standard backbones paired with BBa_J04450.  
 
*Registry standard backbones paired with BBa_J04450.  
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====Kit Plate 5====
 
====Kit Plate 5====
−
<html><img src="https://static.igem.org/mediawiki/parts/d/d1/2015_KP5.jpg" style="float: left; width: 300px; padding: 5px 20px 20px 20px;" /></html>
+
<html><img src="https://static.igem.org/mediawiki/parts/a/a5/2016Distribution_KP5.jpg" style="float: left; width: 300px; padding: 5px 20px 20px 20px;" /></html>
−
DNA Distribution Kit Plate 5 contains selected parts from 2014 submissions, all in pSB1C3. These selected parts were sequence confirmed or ends confirmed. Please note that, in 2014 we received over 2000 samples, so while Kit Plate 5 only contains a selection from this number, you can request parts not in the distribution during the year.
+
[https://parts.igem.org/assembly/plates.cgi?id=4399 DNA Distribution Kit Plate 5 ] contains selected parts from 2014 submissions, all in pSB1C3. These selected parts were sequence confirmed or ends confirmed. Please note that, in 2014 we received over 2000 samples, so while Kit Plate 5 only contains a selection from this number, you can request parts not in the distribution during the year.
 +
<div style="clear: both;"></div>
 +
 
 +
====Kit Plate 6====
 +
<html><img src="https://static.igem.org/mediawiki/parts/f/f3/2016_Distribution_KP6.jpg" style="float: left; width: 300px; padding: 5px 20px 20px 20px;" /></html>
 +
[https://parts.igem.org/assembly/plates.cgi?id=4400 DNA Distribution Kit Plate 6 ] contains selected parts synthesized by Gen9, all in pSB1C3. This plate also includes a small selection of PhytoBrick parts.
 
<div style="clear: both;"></div>
 
<div style="clear: both;"></div>
  
  
−
===Transformation Efficiency Kit===
+
===Competent Cell Test Kit===
  
−
<html><img src="https://static.igem.org/mediawiki/parts/9/97/2014_Distribution_TEK.jpg" style="float: left; width: 300px; padding: 5px 20px 20px 20px;" /></html>
+
<html><img src="https://static.igem.org/mediawiki/parts/a/a4/2016_Distribution_CCTK.jpg" style="float: left; width: 300px; padding: 5px 20px 20px 20px;" /></html>
−
<div class="redbox">Confidence</div> Whether you’re purchasing competent cells or making your own in the lab, you should test their efficiency before you use them with the parts in the DNA Distribution Kit Plates. The Transformation Efficiency Kit is a standardized way to test and calculate the efficiency of your competent cells. If you have issues with using the parts in the Distribution Kit, the first thing we’ll need to know is the efficiency of your competent cells.  
+
<div class="redbox">Confidence</div> Whether you’re purchasing competent cells or making your own in the lab, you should test their efficiency before you start transforming parts in the Distribution Kit. The Competent Cell Test Kit is a standardized way to test and calculate the efficiency of your competent cells. If you have issues with using the parts in the Distribution Kit, the first thing we’ll need to know is the efficiency of your competent cells.  
  
−
See the [[Help:Competent_Cell_Test_Kit | Transformation Efficiency Kit page]] for more in-depth instructions.  
+
See the [[Help:Competent_Cell_Test_Kit | Competent Cell Test Kit page]] for more in-depth instructions.  
 
<br />
 
<br />
−
'' '''Storage:''' The Transformation Efficiency Kit should be stored at 4C or -20C, for longer term storage.''
+
'' '''Storage:''' The Competent Cell Test Kit should be stored at 4C or -20C, for longer term storage.''
 
<div style="clear: both;"></div>
 
<div style="clear: both;"></div>
  
 
===Linearized Plasmid Backbones===
 
===Linearized Plasmid Backbones===
  
−
<html><img src="https://static.igem.org/mediawiki/parts/7/7f/2014_Distribution_LPB.jpg" style="float: left; width: 300px; padding: 5px 20px 20px 20px;" /></html>
+
<html><img src="https://static.igem.org/mediawiki/parts/0/04/2016_Distribution_LPB.jpg" style="float: left; width: 300px; padding: 5px 20px 20px 20px;" /></html>
 
The 2016 Distribution Kit includes a set of four linearized plasmid backbones: pSB1A3, pSB1C3, pSB1T3, and pSB1K3.m1. These plasmid backbones have been prepared via PCR and purified. Prior to ligation the linearized backbones will need to be digested with with EcoRI, PstI and DpnI (DpnI is optional: to cut up original template DNA used to create linearized plasmid backbone), leaving two ends ready to be ligated to a BioBrick part. All 2016 submissions will need to be in pSB1C3, so we recommend using our pSB1C3 linearized backbone for that purpose.  
 
The 2016 Distribution Kit includes a set of four linearized plasmid backbones: pSB1A3, pSB1C3, pSB1T3, and pSB1K3.m1. These plasmid backbones have been prepared via PCR and purified. Prior to ligation the linearized backbones will need to be digested with with EcoRI, PstI and DpnI (DpnI is optional: to cut up original template DNA used to create linearized plasmid backbone), leaving two ends ready to be ligated to a BioBrick part. All 2016 submissions will need to be in pSB1C3, so we recommend using our pSB1C3 linearized backbone for that purpose.  
  
 
See the [[Help:Protocols/Linearized Plasmid Backbones | Linearized Plasmid Backbone protocol page]] for more in-depth instructions on how to use them and make your own. <br />
 
See the [[Help:Protocols/Linearized Plasmid Backbones | Linearized Plasmid Backbone protocol page]] for more in-depth instructions on how to use them and make your own. <br />
 
'' '''Storage:''' The Linearized Plasmid Backbones (pSB1C3/pSB1A3/pSB1T3/pSB1K3: 25ng/ul at 50ul) should be stored at 4C or -20C, for longer term storage''
 
'' '''Storage:''' The Linearized Plasmid Backbones (pSB1C3/pSB1A3/pSB1T3/pSB1K3: 25ng/ul at 50ul) should be stored at 4C or -20C, for longer term storage''
 +
<div style="clear: both;"></div>
 +
 +
===InterLab Measurement Kit===
 +
 +
<html><img src="https://static.igem.org/mediawiki/parts/4/44/2016_Distribution_IMK.jpg" style="float: left; width: 300px; padding: 5px 20px 20px 20px;" /></html>
 +
New for 2016, we are providing teams with the '''InterLab Measurement Kit!''' The InterLab activity is completely optional and is not required for any team.
 +
The kit includes 7 tubes (5 with plasmid DNA and 3 with standard reagents):
 +
 +
See the [[Help:InterLab_Measurement_Kit | InterLab Measurement Kit page]] for more in-depth instructions. <br />
 +
'' '''Storage:''' The InterLab_Measurement_Kit should be stored at room temperature or 4C. <b><font color="red">Do not store below 4C as this causes an IRREVERSIBLE precipitation of the LUDOX solution (ex. -20C)</font></b>''
 
<div style="clear: both;"></div>
 
<div style="clear: both;"></div>
  
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* a box with the iGEM HQ address, for shipping your submission
 
* a box with the iGEM HQ address, for shipping your submission
  
−
See the [[Help:Submission_Kit | 2015 Submission Kit]] for more in-depth instructions.  
+
See the [[Help:Submission_Kit | 2016 Submission Kit]] for more in-depth instructions.  
 
<br />
 
<br />
 
'' '''Storage:''' The 2016 Submission Kit should be stored at room temperature''
 
'' '''Storage:''' The 2016 Submission Kit should be stored at room temperature''
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Before using the DNA plates, you should [[Help:Locating_Parts|search the Registry]] for useful parts, which will also tell you if we have sample in stock, its location (if they're in your 2016 Distribution), requirements, quality control, etc.
 
Before using the DNA plates, you should [[Help:Locating_Parts|search the Registry]] for useful parts, which will also tell you if we have sample in stock, its location (if they're in your 2016 Distribution), requirements, quality control, etc.
  
−
You can also [https://parts.igem.org/assembly/libraries.cgi?id=62 browse the contents] of the 2016 Distribution in their entirety by visiting the [https://parts.igem.org/assembly/libraries.cgi Repository.]
+
 
 +
You can [https://parts.igem.org/assembly/libraries.cgi?id=66 browse the contents] of the 2016 Distribution in their entirety by visiting the [https://parts.igem.org/assembly/libraries.cgi Repository.]
  
 
====Searching====
 
====Searching====
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*The '''[[Help:Locating_Parts|Search Menu]]''' will let you search by text or part name.
 
*The '''[[Help:Locating_Parts|Search Menu]]''' will let you search by text or part name.
 
*It is also possible to see the contents of the Kit Plates in their entirety. You can look at the [https://parts.igem.org/cgi/assembly/libraries.cgi DNA Repositories] section on the main page.
 
*It is also possible to see the contents of the Kit Plates in their entirety. You can look at the [https://parts.igem.org/cgi/assembly/libraries.cgi DNA Repositories] section on the main page.
−
**Click on [https://parts.igem.org/assembly/libraries.cgi?id=62 2015 Distribution] to see the contents of all of your kit plates.  
+
**Click on [https://parts.igem.org/assembly/libraries.cgi?id=66 2016 Distribution] to see the contents of all of your kit plates.  
 
**Click on the 2016 Kit Plate of your choice, which will list all parts by their part name (BBa_..) in a plate along with their quality control information. Or you can click on the small part diagram below each Kit Plate link: "See a summary of the parts in this plate."  
 
**Click on the 2016 Kit Plate of your choice, which will list all parts by their part name (BBa_..) in a plate along with their quality control information. Or you can click on the small part diagram below each Kit Plate link: "See a summary of the parts in this plate."  
 
**These options will show you what is in each well of your plate, however they are not the best way to find specific parts you would like to use.
 
**These options will show you what is in each well of your plate, however they are not the best way to find specific parts you would like to use.
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===Storage===
 
===Storage===
 
*'''DNA Distribution Kit Plates:''' The distribution kit plates are comprised of dried DNA, so they are stable at room temperature. However, once the DNA is resuspended in any of the wells, we recommend either storing the kit plate with its plastic cover in a -20C freezer, or aspirating the rest of the resuspended DNA from the well and keeping it separately in a -20C freezer.
 
*'''DNA Distribution Kit Plates:''' The distribution kit plates are comprised of dried DNA, so they are stable at room temperature. However, once the DNA is resuspended in any of the wells, we recommend either storing the kit plate with its plastic cover in a -20C freezer, or aspirating the rest of the resuspended DNA from the well and keeping it separately in a -20C freezer.
−
*'''Transformation Efficiency Kit:''' The Transformation Efficiency Kit should be stored at 4C or -20C, for longer term storage.
+
*'''Competent Cell Test Kit:''' The Competent Cell Test Kit should be stored at 4C or -20C, for longer term storage.
 
*'''Linearized Plasmid Backbones:''' The Linearized Plasmid Backbones (pSB1C3/pSB1A3/pSB1T3/pSB1K3: 25ng/ul at 50ul) should be stored at 4C or -20C, for longer term storage.
 
*'''Linearized Plasmid Backbones:''' The Linearized Plasmid Backbones (pSB1C3/pSB1A3/pSB1T3/pSB1K3: 25ng/ul at 50ul) should be stored at 4C or -20C, for longer term storage.
 +
*'''InterLab Measurement Kit:''' The InterLab Measurement Kit should be stored at room temperature or 4C. Do not store below 4C (e.g. -20C)''
  
  
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===DNA Kit Plate Instructions===
 
===DNA Kit Plate Instructions===
−
<b>Before you use the DNA in the Distribution Kit Plates, be sure to test the efficiency of your competent cells with the [[#Transformation_Efficiency_Kit | Transformation Efficiency Kit.]]</b><br />
+
<b>Before you use the DNA in the Distribution Kit Plates, be sure to test the efficiency of your competent cells with the [[#Competent_Cell_Test_Kit | Competent Cell Test Kit.]]</b><br />
  
 
To use the DNA in the Distribution Kit, follow these instructions:<br />
 
To use the DNA in the Distribution Kit, follow these instructions:<br />
Line 208: Line 230:
  
 
{{HelpPage/Footer}}
 
{{HelpPage/Footer}}
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__NOEDITSECTION__
 
__NOEDITSECTION__

Latest revision as of 15:28, 14 June 2017


The iGEM 2016 DNA Distribution contains over 1200 high-quality part samples as dried (miniprepped plasmid) DNA. Each sample is QC tested through sequencing and AB test plates.

While there is not enough DNA for 3A Assembly right out of the kit, you can transform a part into competent cells and make your own glycerol stock for future use.

When you receive your Distribution Kit, let us know through twitter (#WelcomeToiGEM). If you're new to the Registry, iGEM, or synthetic biology, you will also want to read through our Help pages before you get started.

Otherwise, read on!


Features of the 2016 DNA Distribution

Quality
In making this distribution, we selected only parts with samples that were sequence confirmed or ends confirmed (long parts). More...

Standards
All parts in Kit Plates 1 - 3, and 5 - 6 are in pSB1C3, the standard shipping plasmid backbone for the iGEM Registry. More...

Confidence
We’ve included the Competent Cell Test Kit, so that you can test the transformation efficiency of your competent cells, before you start using the Distribution Kit Plates. More...

Inspected
All of the Distribution Kit Plates are visually inspected to make sure they contain the DNA needed for transformation.

What's included in the 2016 Distribution

Your 2016 DNA Distribution contains the following:
Green BulletPt.png DNA Distribution Kit Plates 1 - 6
Green BulletPt.png Linearized Plasmid Backbones
Green BulletPt.png Competent Cell Test Kit
Green BulletPt.png InterLab Measurement Kit NEW!
Green BulletPt.png 2016 Part Submission Kit NEW!
Green BulletPt.png iGEM Stickers and Pins!
Green BulletPt.png Sponsor Material from PLOS and IDT NEW!

If there is an issue with your distribution kit, please send an email to hq (at) igem . org


DNA Distribution Kit Plates

Quality
In making this year’s distribution, we selected only parts with samples that were sequence confirmed or ends confirmed (long parts > 1600bp). All parts underwent an additional round of our quality control process: sequencing and antibiotic testing. This has ensured a high quality distribution, in which all parts have a sequence result of Confirmed, Partially Confirmed or Long Part. However, be sure to take a look at the quality control results before using a part! Note: We're still in the process of curating the Distribution, and removing samples that did not meet our requirements!

Storage: The distribution kit plates are comprised of dried DNA with cresol red dye, so they are stable at room temperature. However, once the DNA is resuspended in any of the wells, we recommend either storing the kit plate with its plastic cover in a -20C freezer, or aspirating the rest of the resuspended DNA from the well and keeping it separately in a -20C freezer.

Kit Plates 1 - 3

Standards
In addition to the new parts that have been submitted to the Registry, we have transferred over many of our existing sequence verified parts into pSB1C3, the Registry’s standard shipping plasmid backbone. All parts in DNA Distribution Kit Plates 1 to 3 are in pSB1C3.

Why is this useful?

  • all parts are flanked by the BioBrick prefix and suffix.
  • pSB1C3 has our standard primer sites (VF2 and VR), so you can sequence all parts with the same primer set.
  • pSB1C3 has a high copy origin, improving yields for minipreps
  • pSB1C3 is chloramphenicol resistant, so growing and maintaining parts only requires a single antibiotic

Kit Plate 4

DNA Distribution Kit Plate 4 contains parts in a variety of Registry standard plasmid backbones (pSB1C3, pSB1A3, pSB3K3, etc). On this plate you'll find...

  • Registry standard backbones paired with BBa_J04450.
  • Selected parts from 2013 Submissions.
  • Selected parts featured on the Registry

Kit Plate 5

DNA Distribution Kit Plate 5 contains selected parts from 2014 submissions, all in pSB1C3. These selected parts were sequence confirmed or ends confirmed. Please note that, in 2014 we received over 2000 samples, so while Kit Plate 5 only contains a selection from this number, you can request parts not in the distribution during the year.

Kit Plate 6

DNA Distribution Kit Plate 6 contains selected parts synthesized by Gen9, all in pSB1C3. This plate also includes a small selection of PhytoBrick parts.


Competent Cell Test Kit

Confidence
Whether you’re purchasing competent cells or making your own in the lab, you should test their efficiency before you start transforming parts in the Distribution Kit. The Competent Cell Test Kit is a standardized way to test and calculate the efficiency of your competent cells. If you have issues with using the parts in the Distribution Kit, the first thing we’ll need to know is the efficiency of your competent cells.

See the Competent Cell Test Kit page for more in-depth instructions.
Storage: The Competent Cell Test Kit should be stored at 4C or -20C, for longer term storage.

Linearized Plasmid Backbones

The 2016 Distribution Kit includes a set of four linearized plasmid backbones: pSB1A3, pSB1C3, pSB1T3, and pSB1K3.m1. These plasmid backbones have been prepared via PCR and purified. Prior to ligation the linearized backbones will need to be digested with with EcoRI, PstI and DpnI (DpnI is optional: to cut up original template DNA used to create linearized plasmid backbone), leaving two ends ready to be ligated to a BioBrick part. All 2016 submissions will need to be in pSB1C3, so we recommend using our pSB1C3 linearized backbone for that purpose.

See the Linearized Plasmid Backbone protocol page for more in-depth instructions on how to use them and make your own.
Storage: The Linearized Plasmid Backbones (pSB1C3/pSB1A3/pSB1T3/pSB1K3: 25ng/ul at 50ul) should be stored at 4C or -20C, for longer term storage

InterLab Measurement Kit

New for 2016, we are providing teams with the InterLab Measurement Kit! The InterLab activity is completely optional and is not required for any team. The kit includes 7 tubes (5 with plasmid DNA and 3 with standard reagents):

See the InterLab Measurement Kit page for more in-depth instructions.
Storage: The InterLab_Measurement_Kit should be stored at room temperature or 4C. Do not store below 4C as this causes an IRREVERSIBLE precipitation of the LUDOX solution (ex. -20C)

2016 Submission Kit

For 2016, we are standardizing the part submission shipment: all part submissions must be shipped as dried DNA, in 96 well plate format, and covered with adhesive foil. We've provided a submission kit with all of the materials you'll need to ship your parts.

The Submission Kit includes:

  • (3) 96-well skirted PCR plates
  • (3) universal plate lids
  • (5) adhesive foils
  • bubble wrap
  • a box with the iGEM HQ address, for shipping your submission

See the 2016 Submission Kit for more in-depth instructions.
Storage: The 2016 Submission Kit should be stored at room temperature

Getting Started

Locating a Part in the Distribution

Before using the DNA plates, you should search the Registry for useful parts, which will also tell you if we have sample in stock, its location (if they're in your 2016 Distribution), requirements, quality control, etc.


You can browse the contents of the 2016 Distribution in their entirety by visiting the Repository.

Searching

  • The Catalog of Parts and Devices will allow you to browse parts and devices by various criteria, including function, chassis, standard, etc.
  • The Search Menu will let you search by text or part name.
  • It is also possible to see the contents of the Kit Plates in their entirety. You can look at the DNA Repositories section on the main page.
    • Click on 2016 Distribution to see the contents of all of your kit plates.
    • Click on the 2016 Kit Plate of your choice, which will list all parts by their part name (BBa_..) in a plate along with their quality control information. Or you can click on the small part diagram below each Kit Plate link: "See a summary of the parts in this plate."
    • These options will show you what is in each well of your plate, however they are not the best way to find specific parts you would like to use.

In stock

If you find a part that you would like to use, you need to make sure that a sample of the part is in stock. There are many parts on the Registry that people are still working on, or have decided not to continue working on anymore. This of course means that the DNA is not available in the Registry's Repository. The simplest way to tell whether the part has a sample is to look at the top right of the part's Main Page. If the part has an available sample the top part of the box will be green and say "Sample In stock."

Requesting a part

We've taken care to create a very well-rounded DNA Distribution this spring, however, should you find the part, or parts, that you require are not available in the 2016 DNA Distribution but are available elsewhere in the Registry's Repository, send us an email (hq [AT] igem [DOT] org) in order to request a sample. Include the part name, the plasmid it's located in, the source, and the quality control information, and we'll send it out to you. For more information please see the Requesting Parts page

Using the online QC resources

Here at Registry, we want everyone to take a look at the results of the quality control measures we've taken this year and previous years, in order to make an informed decision when choosing to use a part. We've made sure to update the online repository for the Spring 2016 distribution with our quality control results.

The best way to use our quality control information is to use it on a part by part basis. As you design your project, make sure to check every part that you're interested in for its QC data. After searching for a part in the registry and arriving at its main page, click on the Get This Part link which will take you to the section listing various quality control information for a samples of that part and its location within the registry.

Using the Spring 2016 DNA Distribution

Storage

  • DNA Distribution Kit Plates: The distribution kit plates are comprised of dried DNA, so they are stable at room temperature. However, once the DNA is resuspended in any of the wells, we recommend either storing the kit plate with its plastic cover in a -20C freezer, or aspirating the rest of the resuspended DNA from the well and keeping it separately in a -20C freezer.
  • Competent Cell Test Kit: The Competent Cell Test Kit should be stored at 4C or -20C, for longer term storage.
  • Linearized Plasmid Backbones: The Linearized Plasmid Backbones (pSB1C3/pSB1A3/pSB1T3/pSB1K3: 25ng/ul at 50ul) should be stored at 4C or -20C, for longer term storage.
  • InterLab Measurement Kit: The InterLab Measurement Kit should be stored at room temperature or 4C. Do not store below 4C (e.g. -20C)


DNA Kit Plate Orientation

Locating Your Part from iGEM Videos.

Top view of plates containing dry DNA; red circle indicates well 13H

The foil covers on the (384 well) Kit Plates can be easily punched through with a pipette tip. Unfortunately, the foil cover will also obscure both column and row markings. You can still find your part by correctly orienting the plate using the two notched corners as markers: well A1 is located at the upper left corner of the plate when the long side of the plate with the notched corners is considered the bottom.

Make sure that the two notched corners of the plate are oriented at the BOTTOM of the plate (see the "top view" image on the right for correct orientation)

DNA Kit Plate Instructions

Before you use the DNA in the Distribution Kit Plates, be sure to test the efficiency of your competent cells with the Competent Cell Test Kit.

To use the DNA in the Distribution Kit, follow these instructions:
Note: There is an estimated 2-3ng of DNA in each well, following this protocol, assume that you are transforming with 200-300pg/ul

  1. With a pipette tip, punch a hole through the foil cover into the corresponding well of the part that you want. Make sure you have properly oriented the plate. Do not remove the foil cover, as it could lead to cross contamination between the wells.
  2. Pipette 10uL of dH2O (distilled water) into the well. Pipette up and down a few times and let sit for 5 minutes to make sure the dried DNA is fully resuspended. The resuspension will be red, as the dried DNA has cresol red dye. We recommend that you do not use TE to resuspend the dried DNA.
  3. Transform 1ul of the resuspended DNA into your desired competent cells, plate your transformation with the appropriate antibiotic* and grow overnight.
  4. Pick a single colony and inoculate broth (again, with the correct antibiotic) and grow for 16 hours.
  5. Use the resulting culture to [http://openwetware.org/wiki/Miniprep/Kit-free_high-throughput_protocol miniprep] the DNA AND make your own glycerol stock (for further instruction on making a glycerol see [http://openwetware.org/wiki/Endy:Making_a_long_term_stock_of_bacteria this page]). We recommend using the miniprepped DNA to run QC tests, such as restriction digests and sequencing.

* To know which antibiotics to use, look at the plasmid that the part is in. The naming scheme for plasmids is specifically designed to indicate antibiotic resistance.

Note: There is not enough DNA in each well to perform anything but transformations


Get & Use...

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