BioScaffold Parts

BioScaffold parts are BioBrick parts that contain restriction sites that enable their full or partial removal from a BioBrick assembly.

class α parts: cut outside of scars surrounding the BioScaffold part

class β parts: cut within scars surrounding BioScaffold part

class γ parts: cut only within the BioScaffold part

mixed parts: contain properties of several classes


Part assembly	System operation	Protein expression	Assembly of protein fusions	Part measurement	Screening of part libraries	Building BioBrick vectors	DNA synthesis	Other standards	Archive

Or get some help on plasmid backbones.

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Name	Description	Resistance	Replicon	Copy number	Length
BBa_J70003	Geneart Cloning Plasmid pGA4	A			2961
BBa_J70004	BioBasic Cloning Plasmid pUC57	A			2710
BBa_J70022	GENEART pGA4 plasmid				2978
BBa_K2812001	Coding sequence for trunctated Lysostaphin				738
BBa_K2812003	Coding sequence for trunctated Lysostaphin regulated by T7-promoter				776
BBa_K2812004	Coding sequence for trunctated Lysostaphin fused to His-tagged HlyA				1458
BBa_K2812005	Coding sequence for trunctated Lysostaphin with HlyA and His6-tag regulated by T7-promoter				1496
BBa_K2812006	Coding sequence for Pyocin S5 with HlyA and His6-tag				2217
BBa_K2812007	Coding sequence for Pyocin S5 with HlyA and His6-tag regulated by pBAD-ara promoter				2288
BBa_K5293014	pHREAC_eGFP_ER				10477
pSB1A1	pUC19 with a BioBrick cloning site (Replaced by pSB1A3)	A	pMB1	500-700	2708

Constitutive

If you're interested in constitutively expressing your protein of interest, use one of these plasmids.

There are no parts for this table

If you're interested in constitutively expressing your protein of interest, use one of these plasmids.

There are no parts for this table

If you're interested in constitutively expressing your protein of interest, use one of these plasmids.

There are no parts for this table

There are no parts for this table