Plasmid backbones/Measurement
Part assembly | System operation | Protein expression | Assembly of protein fusions | Part measurement | Screening of part libraries | Building BioBrick vectors | DNA synthesis | Other standards | Archive |
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Some plasmids are designed to facilitate the measurement of different part classes:
Promoters
- Part:BBa_J23101 - A collection of promoters characterized by Chris Anderson. The plasmid backbone from these parts can be used to measure the activity of other promoters based on expression of RFP.
- There is a kit of components available that is described here to measure promoter activity in standard units. The kit makes use of pSB3K3 as the plasmid and BBa_E0240 (GFP) to measure promoter activity.
Inverters
- Part:pSB1A10 - This dual reporter (GFP and RFP) plasmid can be used to measure the transfer curve of inverter devices.