Signalling

Part:BBa_F2620:Experience

Designed by: Barry Canton [bcanton@mit.edu] and Anna Labno [labnoa@mit.edu]   Group: iGEM04_MIT   (2004-08-09)
Revision as of 21:38, 14 April 2008 by Austin (Talk | contribs)

Input: 3OC6HSL
Output: PoPS

tetR
R0040		LuxR
I0462		lux pR
R0062
 BBa F2620Icon.png

Endy Lab Characterization

We (Barry Canton, Anna Labno, Drew Endy) have measured four characteristics of BBa_F2620. Protocols and results for the four characteristics are linked below. For the purposes of characterization, we connected BBa_E0240 downstream of BBa_F2620 to make the composite part, BBa_T9002. This allowed us to measure the PoPS output from BBa_F2620 indirectly via the fluorescence output of BBa_E0240. Data processing is described here. The data resulting from these experiments is summarized on the paper datasheet: (.ppt, .pdf).

  1. Transfer Function
  2. Specificity
  3. Response time
  4. Stability

Leakiness

See Part:BBa_I13556 for more characterization of F2620 upstream of mCherry instead of GFP. In particular, it was found that when grown in LB, there was strong leaky expression of mCherry in the absence of AHL. There was no leaky expression when grown in EZ rich medium. --Austin 17:11, 6 September 2007 (EDT)

F2620 in front of GFP and F2620 in front of lacZa were also both grown up in LB to stationary phase (without AHL) and both of these in addition to the mCherry also showed leaky expression not present when grown in EZ media. --Austin 17:38, 14 April 2008 (EDT)

User Reviews

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Antiquity

This review comes from the old result system and indicates that this part worked in some test.

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