RFC25 "Freiburg standard" parts

RFC25 BioBrick parts

Fluorescent proteins

Fluorescent proteins
Protein	Color	BioBrick	Excitation [nm]	Emission [nm]	Quantum Yield
Cerulean	Cyan	BBa_I757009	433	475	0.62
GFP	Green	BBa_K648013	484	507	0.60
GFP	Green	BBa_K863120	484	507	0.60
GFP	Green	BBa_K823039	484	507	0.60
sfGFP	Green	BBa_K1321337	485	510	0.65
Dronpa (photoactivatable)	Green	BBa_K1680006	503	518	0.85
mVenus	Yellow	BBa_I757008	515	528	0.57
sYFP2	Yellow	BBa_K1159301	514	527	0.61
eCFP	Cyan	BBa_K1159302	439	476	0.40
mRFP	Red	BBa_K1351021	584	607	0.25
mRFP	Red	BBa_K1615089	584	607	0.25
mKate2	Red	BBa_K823029	588	633

Values for the fluorescent proteins were taken from http://www.microscopyu.com/articles/livecellimaging/fpintro.html.

Luciferases

Luciferases
Protein	BioBrick	Size [kDa]
Firefly luciferase	BBa_K525999	61
Renilla luciferase		36
NanoLuc luciferase	BBa_K1159001	19
Gaussia luciferase

Linker domains

Linker
Protein	Sequence	BioBrick	Length [residues]
Short Linker	(Gly-Gly-Ser-Gly)	BBa_K243004BBa_I757009	4
Middle Linker	2x (Gly-Gly-Ser-Gly)	BBa_K243005BBa_I757009	8
Long Linker	3x (Gly-Gly-Ser-Gly)	BBa_K243006BBa_I757009	12

Catalytic proteins

Catalytic proteins
Erythromycin Esterase Type II	BBa_K1159000
Laccase	BBa_K1159002
Laccase TVEL5	BBa_K1615067
TEV Protease	BBa_K1159100
Thermonuclease	BBa_K1159105
Heroin Esterase	BBa_K1615045
CRE recombinase	BBa_K1680007
Monoamine Oxidase A	BBa_K1615022
Endo-β-Galactosidase	BBa_K1483001
α-Galactosidase	BBa_K1483002
Acetylgalactosamindase	BBa_K1483000
2-oxoglutarate oxygenase/decarboxylase	BBa_K1065000

Transcription regulators

Transcription regulators
VP16	BBa_K1150001

Split proteins

Split proteins
TEV Protease	BBa_K1159101	BBa_K1159102
SpyCatcher/SpyTag	BBa_K1159200	BBa_K1159201

Binding proteins

Binding proteins
Fluorescein binding Anticalin	BBa_K1159003
Digoxigenin binding Anticalin	BBa_K243003
HER2 binding DARPin	BBa_K404302
HER2 binding Affibody	BBa_K404302
Nickel Binding Protein	BBa_K1321009
Cellulose binding Domain	BBa_K863110

Affinity tags

Affinity tags
HA tag	BBa_K1150016
His6-tag	BBa_I757013
SpyTag	BBa_K1159020
Strep-tag II as N-part	BBa_K549018
Strep-tag II	BBa_J18916
cMyc-tag	BBa_K823036
3x FLAG tag	BBa_K823034