Tag

Part:BBa_K549018

Designed by: Franziska Haefele   Group: iGEM11_LMU-Munich   (2011-09-16)


STREP-tag improved

This part is an improvement to the part K157012. It is constructed in the Freiburg-standard with the restriction sites NgoMIV and AgeI. This tag can be fused to proteins in order to purificate this protein.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 15
    Illegal SpeI site found at 57
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 57
    Illegal NotI site found at 6
    Illegal NotI site found at 64
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 15
    Illegal SpeI site found at 57
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 15
    Illegal SpeI site found at 57
    Illegal AgeI site found at 47
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None