Tag
StrepII

Part:BBa_J18916

Designed by: Raik Gruenberg   Group: Affiliates   (2010-01-26)


Strep tag II

Protein purification tag. Bound by Strepavidine (optimized: strepTactin(tm)) and eluted with biotin. Allows for mild and efficient elution.

SEE ALSO:

  • novagen.com, gehealthcare.com (former amersham) for purification protocols
  • BBa_I757014, StrepTag II from Freiburg iGem team, has three codon variations

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//proteindomain/affinity
Parameters
None