Coding

Part:BBa_K1159101

Designed by: Dong-Jiunn Jeffery TRUONG   Group: iGEM13_TU-Munich   (2013-09-14)

N-terminal half of TEV Protease S219V mutant (for Split-TEV-Protease applications) in RFC[25]

N-terminal half of the TEV Protease (autolysis resistant S219V mutant) can be used for Split-TEV-Protease applications where autolysis of the reconstituted TEV Protease is not wanted. The necessary C-terminal half can be found under BBa_K1159102. This part is flanked by RFC[25] pre- and suffix for further protein fusions.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 316

Protein data table for BioBrick BBa_ automatically created by the BioBrick-AutoAnnotator version 1.0
Nucleotide sequence in RFC 25, so ATGGCCGGC and ACCGGT were added (in italics) to the 5' and 3' ends: (underlined part encodes the protein)
 ATGGCCGGCGGAGAAAGC ... TTCCAAACTACCGGT
 ORF from nucleotide position -8 to 360 (excluding stop-codon)
Amino acid sequence: (RFC 25 scars in shown in bold, other sequence features underlined; both given below)
1 
101 		MAGGESLFKGPRDYNPISSTICHLTNESDGHTTSLYGIGFGPFIITNKHLFRRNNGTLLVQSLHGVFKVKNTTTLQQHLIDGRDMIIIRMPKDFPPFPQK
LKFREPQREERICLVTTNFQTTG*
Sequence features: (with their position in the amino acid sequence, see the list of supported features)
Factor Xa cleavage site: 80 to 83
Amino acid composition:
Ala (A)1 (0.8%)
Arg (R)8 (6.5%)
Asn (N)7 (5.7%)
Asp (D)5 (4.1%)
Cys (C)2 (1.6%)
Gln (Q)6 (4.9%)
Glu (E)5 (4.1%)
Gly (G)11 (8.9%)
His (H)5 (4.1%)
Ile (I)10 (8.1%)
Leu (L)11 (8.9%)
Lys (K)7 (5.7%)
Met (M)3 (2.4%)
Phe (F)9 (7.3%)
Pro (P)8 (6.5%)
Ser (S)6 (4.9%)
Thr (T)13 (10.6%)
Trp (W)0 (0.0%)
Tyr (Y)2 (1.6%)
Val (V)4 (3.3%)
Amino acid counting
Total number:123
Positively charged (Arg+Lys):15 (12.2%)
Negatively charged (Asp+Glu):10 (8.1%)
Aromatic (Phe+His+Try+Tyr):16 (13.0%)
Biochemical parameters
Atomic composition:C624H985N177O178S5
Molecular mass [Da]:13975.1
Theoretical pI:9.56
Extinction coefficient at 280 nm [M-1 cm-1]:2980 / 3105 (all Cys red/ox)
Plot for hydrophobicity, charge, predicted secondary structure, solvent accessability, transmembrane helices and disulfid bridges 
Codon usage
Organism:E. coliB. subtilisS. cerevisiaeA. thalianaP. patensMammals
Codon quality (CAI):good (0.67)good (0.69)good (0.68)good (0.76)excellent (0.82)good (0.68)
Alignments (obtained from PredictProtein.org)
SwissProt:P04517 (100% identity on 118 AAs), O36979 (64% identity on 117 AAs)
TrEML:Q000U0 (100% identity on 118 AAs), G9IZP9 (97% identity on 118 AAs)
PDB:1lvm (100% identity on 118 AAs), 1q31 (100% identity on 115 AAs), 1lvb (98% identity on 111 AAs)
Predictions (obtained from PredictProtein.org)
Subcellular Localization (reliability in brackets)
Archaea:secreted (100%)
Bacteria:cytosol (40%)
Eukarya:mitochondria (14%)
Gene Ontology (reliability in brackets)
Molecular Function Ontology: -
Biological Process Ontology:GO:0006508 (40%), GO:0019079 (40%)
 
Predicted features:
Disulfid bridges: -
Transmembrane helices: -
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Categories
Parameters
None