RFC25 "Freiburg standard" parts
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< RFC25 assembly standard description
Contents
RFC25 BioBrick parts
RFC25 compatible BioBrick backbones
For the cloning of RFC25 BioBricks into the Parts Registry compatible backbone the plasmid pSB1C3-RFP_Generator (BBa_K801100) can be used.
Fluorescent proteins
| Fluorescent proteins (click for all RFC25 parts) | |||||
|---|---|---|---|---|---|
| Protein | Color | BioBrick | Excitation [nm] | Emission [nm] | Quantum Yield |
| Cerulean | Cyan | BBa_I757009 | 433 | 475 | 0.62 |
| eCFP | Cyan | BBa_K1159302 | 439 | 476 | 0.40 |
| GFP | Green | BBa_K648013 | 484 | 507 | 0.60 |
| GFP | Green | BBa_K863120 | 484 | 507 | 0.60 |
| GFP | Green | BBa_K823039 | 484 | 507 | 0.60 |
| sfGFP | Green | BBa_K1321337 | 485 | 510 | 0.65 |
| Dronpa (photoactivatable) | GreenYellow | BBa_K1680006 | 503 | 518 | 0.85 |
| mVenus | Yellow | BBa_I757008 | 515 | 528 | 0.57 |
| sYFP2 | Yellow | BBa_K1159301 | 514 | 527 | 0.61 |
| mRFP | Red | BBa_K1351021 | 584 | 607 | 0.25 |
| mRFP | Red | BBa_K1615089 | 584 | 607 | 0.25 |
| mKate2 | Red | BBa_K823029 | 588 | 633 | 0.4 |
| Values for the fluorescent proteins were taken from [http://www.microscopyu.com/articles/livecellimaging/fpintro.html| microscopyu.com]. | |||||
Luciferases
| Luciferases (click for all RFC25 parts) | |||||
|---|---|---|---|---|---|
| Protein | BioBrick | Size [kDa] | |||
| Firefly luciferase | BBa_K525999 | 61 | |||
| Renilla luciferase | not available yet | 36 | |||
| NanoLuc luciferase | BBa_K1159001 | 19 | |||
| Gaussia luciferase | not available yet | ||||
Transmembrane domains
Optogenetic parts
Linker domains
| Linker (click for all RFC25 parts) | |||||
|---|---|---|---|---|---|
| Protein | Sequence | BioBrick | Length [residues] | ||
| Short Linker | (Gly-Gly-Ser-Gly) | BBa_K243004 | 4 | ||
| Middle Linker | 2x (Gly-Gly-Ser-Gly) | BBa_K243005 | 8 | ||
| Long Linker | 3x (Gly-Gly-Ser-Gly) | BBa_K243006 | 12 | ||
Catalytic proteins
| Catalytic proteins (click for all RFC25 parts) | |||||
|---|---|---|---|---|---|
| Erythromycin Esterase Type II | BBa_K1159000 | ||||
| Laccase | BBa_K1159002 | ||||
| Laccase TVEL5 | BBa_K1615067 | ||||
| TEV Protease | BBa_K1159100 | ||||
| Thermonuclease | BBa_K1159105 | ||||
| Heroin Esterase | BBa_K1615045 | ||||
| CRE recombinase | BBa_K1680007 | ||||
| Monoamine Oxidase A | BBa_K1615022 | ||||
| Endo-β-Galactosidase | BBa_K1483001 | ||||
| α-Galactosidase | BBa_K1483002 | ||||
| Acetylgalactosamindase | BBa_K1483000 | ||||
| 2-oxoglutarate oxygenase/decarboxylase | BBa_K1065000 | ||||
Transcription regulators
| Transcription regulators (click for all RFC25 parts) | |||||
|---|---|---|---|---|---|
| VP16 | BBa_K1150001 | ||||
Split proteins
| Split proteins (click for all RFC25 parts) | |||||
|---|---|---|---|---|---|
| TEV Protease | BBa_K1159101 | BBa_K1159102 | |||
| SpyCatcher/SpyTag | BBa_K1159200 | BBa_K1159201 | |||
Binding proteins
| Binding proteins (click for all RFC25 parts) | |||||
|---|---|---|---|---|---|
| Fluorescein binding Anticalin | BBa_K1159003 | ||||
| Digoxigenin binding Anticalin | BBa_K243003 | ||||
| HER2 binding DARPin | BBa_K404302 | ||||
| HER2 binding Affibody | BBa_K404302 | ||||
| Nickel Binding Protein | BBa_K1321009 | ||||
| Cellulose binding Domain | BBa_K863110 | ||||
Protease cleavage sites
| Protease cleavage sites (click for all RFC25 parts) | |||||
|---|---|---|---|---|---|
| Name | Sequence | BioBrick | |||
| TEV cleavage site | G-ENLYFQS-G | BBa_K1159318 | |||
Affinity tags
| Affinity tags (click for all RFC25 parts) | |||||
|---|---|---|---|---|---|
| HA tag | BBa_K1150016 | ||||
| His6-tag | BBa_I757013 | ||||
| SpyTag | BBa_K1159020 | ||||
| Strep-tag II as N-part | BBa_K549018 | ||||
| Strep-tag II | BBa_J18916 | ||||
| cMyc-tag | BBa_K823036 | ||||
| 3x FLAG tag | BBa_K823034 | ||||