Tag
His

Part:BBa_I757013

Designed by: Freiburg iGEM 2007 team   Group: iGEM07_Freiburg   (2007-10-24)

His affinity tag

  • His-tag
  • fusion to proteins facilitates detection, purification, immobilization
  • NgoMIV / AgeI protein fusion part
  • iGEM Team Freiburg 2007
  • synthetic DNA by GeneArt
  • Part in pGA4 vector (AmpR, ColEI ori)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal AgeI site found at 28
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//proteindomain/affinity
Parameters
None