Part:BBa_F2620

Input: 3OC6HSL Output: PoPS

tetR R0040 LuxR I0462 lux pR R0062

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Description
A transcription factor [LuxR] (BBa_C0062) that is active in the presence of cell-cell signaling molecule 3OC₆HSL is controlled by a tetR regulatable operator (BBa_R0040). Device input is 3OC₆HSL. Device output is PoPS from a LuxR-regulated operator. If used in a cell containing TetR then a second input signal such as [http://openwetware.org/wiki/ATc aTc] can be used to produce a logical AND function.

Characteristics
Input Swing:	0.1 - 1000 nM 3OC6HSL, exogenous	L/H Response time:	9.5 mins (50% of max. output)
Output Swing:	21±3 - 590±9 GFP molecules cfu-1 s-1	Genetic Stability:	>92/74 generations (Low/High Input)
Switch Point:	1E-8 M 3OC6HSL, exogenous	Performance Stability:	>92/74 generations (Low/High Input)

Transfer Function	Specificity
Response time	Stability

Demand
Translation Demand: (Low/High Input)	5040/141600 charged tRNA cfu-1 s-1 336/9440 ribosomes sequestered cfu-1	Growth Rate:	54/59 min doubling time (Low/High Input)

Compatibility
Chassis: Device has been shown to work in BBa_V1000,BBa_V1001,BBa_V1002
Plasmids: Device has been shown to work on pSB3K3 and pSB1A3
Devices: Device has been shown to work with BBa_E0430, BBa_E0434, BBa_E0240
Crosstalk with systems containing BBa_C0040.
Cell-Cell Signaling Systems: Crosstalk with devices using 3OC₆HSL, C6HSL, C7HSL, C8HSL, C10HSL.