Part:BBa_F2620

Description
A transcription factor [LuxR] (BBa_C0062) that is active in the presence of cell-cell signaling molecule 3OC₆HSL is controlled by a tetR regulatable operator (BBa_R0040). Device input is 3OC₆HSL. Device output is PoPS from a LuxR-regulated operator. If used in a cell containing TetR then a second input signal such as [http://openwetware.org/wiki/ATc aTc] can be used to produce a logical AND function.

Characteristics Input Swing: 0.1 - 1000 nM 3OC6HSL, exogenous Output Swing: 13.6 - 490.2 GFP molecules/s.cfu Switch Point: 6 nM 3OC6HSL, exogenous L/H Response time: 25 mins

Key Subparts BBa_R0040: TetR-regulated operator BBa_C0062: luxR ORF BBa_R0062: LuxR-regulated operator

Transfer Function	Specificity
Response time	Stability

Demand Transcription Demand: ## NTP per second ## polymerases sequestered Translation Demand: # charged tRNA per second # ribosomes sequestered

Stability Genetic Stability: 92/74 generations (Low/High Input) Performance Stability: 92/74 generations (Low/High Input)

Compatibility
Chassis: Device has been shown to work in BBa_V1002, MG1655 and BBa_V1001
Plasmid: Device has been shown to work on pSB3K3 and pSB1A3
Devices: Device has been shown to work with BBa_E0430,BBa_E0434,BBa_E0240
Crosstalk with devices using 3OC₆HSL or add in the other molecule names here. Crosstalk with systems containing TetR (BBa_C0040)
Characterization Conditions
Device output was measured using the composite part BBa_T9002, ([BBa_F2620].[BBa_E0240]). The characterization platform was BioBrick plasmidpSB3K3 with E. coli MG1655 as chassis. Cultures were grown in [http://openwetware.org/wiki/Endy:M9_media/supplemented supplemented M9 media] at 37C. Full characterization protocols can be found here.