Generator

Part:BBa_K325909

Designed by: Theo Sanderson and Will Handley   Group: iGEM10_Cambridge   (2010-10-23)
Revision as of 11:07, 24 October 2010 by PM (Talk | contribs)

Input: L-Arabinose
Output: Light

pBad/araC
I0500
PoPS to Light
Cambridge-Eglowli.png

Part Main Page        Arabinose -> Light        H-NS mutants        Add Data       


Description
G28 !

THIS PAGE IS CURRENTLY BEING UPDATED.


Performance

Experiment1 Characteristic1 Value1
Transfer Function Maximum Output 6.6 PoPS cell-1
Hill coefficient 1.6
Switch Point 1.5E-9 M 3OC6HSL, exogenous
Response time: <1 min
Input compatibility Strong response to 3OC6HSL, C6HSL , C7HSL, 3OC8HSL, C8HSL
Weak response to C4HSL, C10HSL, C12HSL
Stability Genetic Stability
(Low/High Input)
>92/>56 generations
Performance Stability
(Low/High Input)
>92/>56 generations
Demand Internal Demand
(Low/High Input)
Not measured
Transcriptional output demand:
(Low/High Input)
Nt = length of downstream transcript in nucleotides
(0/6xNt) nucleotides cell-1 s-1
(0/1.5E-1xNt) RNAP cell-1
Growth Rate
(Low/High Input)
54/59 min Doubling time

1Measured by the [http://2010.igem.org/Team:Cambridge Cambridge iGEM team 2010]

Compatibility
Chassis: Device has been shown to work in Top 10 (Invitrogen)
Plasmids: Device has been shown to work on pSB1C3


References
[http://www.jstor.org/stable/4449975 [1]:] J. Slock, (1995) Transformation Experiments Usibg Bioluminescence Genes of Vibrio fischeri,The American Biology Teacher, 57, 225-227.

[http://www.nature.com/nature/journal/v440/n7082/abs/nature04542.html [2]:] T. Nakatsu et al. (2006) Structural Basis for the spectral difference in luciferase bioluminescence, Nature 440(16), 372-376.

[http://www.ncbi.nlm.nih.gov/pubmed/11457857 [3]:] K. Gomi and N. Kajiyama, (2001) Oxyluciferin, a Luminescence Product of Firefly Luciferase, Is Enzymatically Regenerated into Luciferin, The Journal of Biological Chemistry, 276(39), 36508-36513.
[edit]
Categories
Parameters
outputLight
positive_regulatorsL-arabinose