Difference between revisions of "Plasmid backbones/Other standards"
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Revision as of 23:57, 4 February 2009

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Plasmid backbones/Other standards
Part assembly System operation Protein expression Assembly of protein fusions Part measurement Screening of part libraries Building BioBrick vectors DNA synthesis Other standards Archive
Or get some help on plasmid backbones.

Silver assembly standard plasmid backbones

Please see plasmid backbones for protein fusions

Freiburg assembly standard plasmid backbones

Please see plasmid backbones for protein fusions

Berkeley assembly standard plasmid backbones

Researchers at UC Berkeley have developed the BglBrick assembly standard, or Assembly standard 21, based on idempotent assembly with BamHI and BglII restriction enzymes. In a nutshell, most parts look like this: 

        Prefix                        Suffix
5' GAATTC atg AGATCT ...part... GGATCC taa CTCGAG 3'
   EcoRI      BglII             BamHI   *   XhoI

Fusing two parts leaves the following scar: 

5' [part A] GGATCT [part B] 3'
             G  S

Note, however, that Assembly standard 20 is intended as a minimal physical assembly standard, and only those features needed for interconversion of BglBrick assembly standard plasmids are formally defined. Therefore, atg and taa spacers are not core definitions of the standard.

See [http://openwetware.org/wiki/The_BioBricks_Foundation:Standards/Technical/Formats The BioBricks Foundation wiki] for a discussion and comparison of different technical standards.


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Lim assembly standard plasmid backbones

Students in Wendell Lim's lab have developed a new assembly standard. See [http://2008.igem.org/Everything_you_ever_wanted_to_know_about_AarI the 2008 UCSF iGEM team wiki] for more details. Note these plasmid backbones are primarily intended for use when working with yeast.


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SergioPeisajovichPhoto.jpg AndrewHorowitzPhoto.jpg Sergio Peisajovich and Andrew Horowitz, from Wendell Lim's lab, developed several of the yeast plasmid backbones as an instructor of the 2008 UCSF iGEM team.

References

<biblio>

  1. Sikorski pmid=2659436

</biblio>