Difference between revisions of "Terminators/test"

Revision as of 20:50, 15 February 2013

Test page for creating new terminator catalog pages


Forward terminators	Bidirectional terminators	Reverse terminators	Yeast terminators	Eukaryotic terminators	Help!

E. coli terminators

There are several E. coli transcriptional terminators available via the Registry. The most commonly used type of terminator is a forward terminator. When placed downstream of a genetic part that is usually transcribed, a forward transcriptional terminator will cause transcription to abort. However, in general, most transcriptional terminators will not terminate transcription with 100% efficiency. Some RNA polymerases will continue transcribing "through" a transcriptional terminator.

There are also bidirectional transcriptional terminators. Such terminators will usually cause transcription to terminate on both the forward and reverse strand. Again, however, the efficiency of transcriptional termination is not 100% and will often differ in the forward and reverse direction.

Finally, there are also reverse transcriptional terminators that terminate transcription on the reverse strand only.

Forward terminators

*More...*
Name	Description	Direction	Efficiency Fwd. Rev.		Chassis	Length
BBa_B0010	T1 from E. coli rrnB	Forward				80
BBa_B0012	TE from coliphageT7	Forward	0.309[CC]	-0.368[CC]		41
BBa_B0013	TE from coliphage T7 (+/-)	Forward	0.6[CC]	-1.06[CC]		47
BBa_B0015	double terminator (B0010-B0012)	Forward	0.984[CC] 0.97[JK]	0.295[CC] 0.62[JK]	E.coli	129
BBa_B0017	double terminator (B0010-B0010)	Forward				168
BBa_B0053	Terminator (His)	Forward				72
BBa_B0055	-- No description --					78
BBa_B1002	Terminator (artificial, small, %T~=85%)	Forward	0.98[CH]			34
BBa_B1003	Terminator (artificial, small, %T~=80)	Forward	0.83[CH]			34
BBa_B1004	Terminator (artificial, small, %T~=55)	Forward	0.93[CH]			34
BBa_B1005	Terminator (artificial, small, %T~=25%	Forward	0.86[CH]			34
BBa_B1006	Terminator (artificial, large, %T~>90)	Forward	0.99[CH]			39
BBa_B1010	Terninator (artificial, large, %T~<10)	Forward	0.95[CH]			40
BBa_I11013	Modification of biobricks part BBa_B0015					129
BBa_I51003	-- No description --					110
BBa_J61048	[rnpB-T1] Terminator	Forward	0.98[JCA]			113
BBa_K1392970	Terminator+Tetr Promoter+T4 Endolysin					623
BBa_K1486001	Arabinose promoter + CpxR	Forward			Escherichia coli	1924
BBa_K1486005	Arabinose promoter + sfGFP-CpxR [Cterm]	Forward			Escherichia coli	2668
BBa_K1486009	CxpR & Split IFP1.4 [Nterm + Nterm]	Forward			Escherichia coli	3726
BBa_K780000	Terminator for Bacillus subtilis					54
BBa_K864501	T22, P22 late terminator	Forward				42
BBa_K864600	T0 (21 imm) transcriptional terminator	Forward	0.97			52
BBa_K864601	Lambda t1 transcriptional terminator	Forward	0.97			53

Bidirectional terminators

*More...*
Name	Description	Direction	Efficiency Fwd. Rev.		Length
BBa_B0011	LuxICDABEG (+/-)	Bidirectional	0.419[CC]/0.95[JK]	0.636[CC]/0.86[JK]	46
BBa_B0014	double terminator (B0012-B0011)	Bidirectional	0.604[CC]/0.96[JK]	0.86[JK]	95
BBa_B0021	LuxICDABEG (+/-), reversed	Bidirectional	0.636[CC]/0.86[JK]	0.419[CC]/0.95[JK]	46
BBa_B0024	double terminator (B0012-B0011), reversed	Bidirectional	0.86[JK]	0.604[CC]/0.96[JK]	95
BBa_B0050	Terminator (pBR322, +/-)	Bidirectional			33
BBa_B0051	Terminator (yciA/tonA, +/-)	Bidirectional			35
BBa_B1001	Terminator (artifical, small, %T~=90)	Bidirectional	0.81[CH]		34
BBa_B1007	Terminator (artificial, large, %T~=80)	Bidirectional	0.83[CH]		40
BBa_B1008	Terminator (artificial, large, %T~=70)	Bidirectional			40
BBa_B1009	Terminator (artificial, large, %T~=40%)	Bidirectional			40
BBa_K187025	terminator in pAB, BioBytes plasmid				60
BBa_K259006	GFP-Terminator	Bidirectional	0.604[CC]/0.96[JK]	0.86[JK]	823

Reverse terminators

*More...*
Name	Description	Direction	Efficiency Fwd. Rev.		Length
BBa_B0020	Terminator (Reverse B0010)	Reverse			82
BBa_B0022	TE from coliphageT7, reversed	Reverse	-0.368[CC]	0.309[CC]	41
BBa_B0023	TE from coliphage T7, reversed	Reverse	-1.06[CC]	0.6[CC]	47
BBa_B0025	double terminator (B0015), reversed	Reverse	0.295[CC]/0.62[JK]	0.984[CC]/0.97[JK]	129
BBa_B0052	Terminator (rrnC)	Forward			41
BBa_B0060	Terminator (Reverse B0050)	Bidirectional			33
BBa_B0061	Terminator (Reverse B0051)	Bidirectional			35
BBa_B0063	Terminator (Reverse B0053)	Reverse			72

	Reshma Shetty, as a member of the 2003 MIT IAP class in synthetic biology, designed transcriptional terminators BBa_B0011-BBa_B0016.		Cassie Huang, as a masters student in Tom Knight's lab, designed, constructed and characterized transcriptional terminators BBa_B1001-BBa_B1010.
	Caitlin Conboy, as a member of Drew Endy's lab, constructed and characterized transcriptional terminators BBa_B0021-BBa_B0025 and characterized transcriptional terminators BBa_B0011-BBa_B0015.		Chris Anderson, a professor of bioengineering at UC Berkeley, designed, constructed and characterized terminator BBa_J61048.

Yeast terminators

Here are all the yeast terminators available. As you can see, there are only a couple available, so please design, construct, and characterize new ones and submit them to the Registry!

*More...*
Name	Description	Direction	Chassis	Length
BBa_J63002	ADH1 terminator from S. cerevisiae	Forward		225
BBa_K110012	STE2 terminator	Forward		123
BBa_K1462070	cyc1			250
BBa_K1486025	ADH1 Terminator	Forward	Saccharomyces Cerevisiae	188
BBa_K2314608	Tmini is a very short terminator in yeast. It's only 68bp in length but has a good performance.			68
BBa_K2637012	ADH1 Terminator		S. cerevisiae	335
BBa_K2637014	TEF1 Terminator		S. cerevisiae	476
BBa_K2637016	PGK1 Terminator		S. cerevisiae	285
BBa_K2637017	CYC1 terminator			261
BBa_K3768004	CPS1 Terminator for S. cerevisiae			191
BBa_K392003	yeast ADH1 terminator			129
BBa_K4947020	Yeast terminator with I-SceI restriction site			50
BBa_K801011	TEF1 yeast terminator			507
BBa_K801012	ADH1 yeast terminator			349
BBa_Y1015	CycE1			252

Caroline Ajo-Franklin developed the yeast terminator BBa_J63002 as a post-doc in Pam Silver's lab.

Eukaryotic terminators

Here are all the eukaryotic, including yeast, terminators available. As you can see, there are only a handful available, so please design, construct, and characterize new ones and submit them to the Registry!

*More...*
Name	Description	Direction	Chassis	Length
BBa_J52016	eukaryotic -- derived from SV40 early poly A signal sequence	Forward		238
BBa_J63002	ADH1 terminator from S. cerevisiae	Forward		225
BBa_K110012	STE2 terminator	Forward		123
BBa_K1159307	35S Terminator of Cauliflower Mosaic Virus (CaMV)			217
BBa_K1462070	cyc1			250
BBa_K1484215	nopaline synthase terminator			293
BBa_K1486025	ADH1 Terminator	Forward	Saccharomyces Cerevisiae	188
BBa_K2314608	Tmini is a very short terminator in yeast. It's only 68bp in length but has a good performance.			68
BBa_K2637012	ADH1 Terminator		S. cerevisiae	335
BBa_K2637014	TEF1 Terminator		S. cerevisiae	476
BBa_K2637016	PGK1 Terminator		S. cerevisiae	285
BBa_K3758200	psbA 3-UTR (Nicotiana tabacum)			93
BBa_K392003	yeast ADH1 terminator			129
BBa_K404108	hGH terminator			481
BBa_K404116	hGH_[AAV2]-right-ITR			632
BBa_K4213000	Plant Thiamine Pyrophosphate Riboswitch			675
BBa_K4235020	SV40 poly(A) signal		Spodoptera Frugiperda	135
BBa_K4947020	Yeast terminator with I-SceI restriction site			50
BBa_K678012	SV40 poly A, terminator for mammalian cells			139
BBa_K678018	hGH poly A, terminator for mammalian cells			635
BBa_K678019	BGH poly A, mammalian terminator			233
BBa_K678036	trpC terminator for Aspergillus nidulans			759
BBa_K678037	T1-motni, terminator for Aspergillus niger			1006
BBa_K678038	T2-motni, terminator for Aspergillus niger			990
BBa_K678039	T3-motni, terminator for Aspergillus niger			889
BBa_K801011	TEF1 yeast terminator			507
BBa_K801012	ADH1 yeast terminator			349
BBa_Y1015	CycE1			252

Monika Ciglic constructed this eukaryotic terminator as a member of the [http://2006.igem.org/Ljubljana,_Slovenia_2006 2006 Slovenija iGEM team].

_

@@ Line 1: / Line 1: @@
 Test page for creating new terminator catalog pages
+[[Terminators|< Back to Terminators]]
+{|align="center"
+|align='center' width=100px|{{Click || image=TerminatorsForward.png | link=Terminators/Catalog#Forward terminators |width=50px | height=50px }}
+|align='center' width=100px|{{Click || image=TerminatorsBidirectional.png | link=Terminators/Catalog#Bidirectional terminators |width=50px | height=50px }}
+|align='center' width=100px|{{Click || image=TerminatorsReverse.png | link=Terminators/Catalog#Reverse terminators |width=50px | height=50px }}
+|align='center' width=100px|{{Click || image=TerminatorsYeast.png | link=Terminators/Catalog#Yeast terminators |width=50px | height=50px }}
+|align='center' width=100px|{{Click || image=TerminatorsEukaryote.png | link=Terminators/Catalog#Eukaryotic terminators |width=50px | height=50px }}
+|align='center' width=100px|{{Click || image=Plasmidhelpbutton.png | link=Help:Terminators |width=50px | height=50px }}
+|-
+|align='center' width=150px|'''[[Terminators/Catalog#Forward terminators|Forward terminators]]'''
+|align='center' width=150px|'''[[Terminators/Catalog#Bidirectional terminators|Bidirectional terminators]]'''
+|align='center' width=150px|'''[[Terminators/Catalog#Reverse terminators|Reverse terminators]]'''
+|align='center' width=150px|'''[[Terminators/Catalog#Yeast terminators|Yeast terminators]]'''
+|align='center' width=150px|'''[[Terminators/Catalog#Eukaryotic terminators|Eukaryotic terminators]]'''
+|align='center' width=150px|'''[[Help:Terminators|Help!]]'''
+|}
+----
+<html>
+<style>
+#assembly_plasmid_table {margin-left:auto;margin-right:auto; border: 3px solid #444444;}
+#assembly_plasmid_table td {background-color:#eeeeee; padding: 5px; font-size: 12px;}
+#assembly_plasmid_table th {background-color: #aaaaaa;padding: 5px; font-size:14px;}
+</style>
+</html>
+==''E. coli'' terminators==
+There are several ''E. coli'' transcriptional terminators available via the Registry.  The most commonly used type of terminator is a forward terminator.  When placed downstream of a genetic part that is usually transcribed, a forward transcriptional terminator will cause transcription to abort.  However, in general, most transcriptional terminators will not terminate transcription with 100% efficiency.  Some RNA polymerases will continue transcribing "through" a transcriptional terminator.
+There are also bidirectional transcriptional terminators.  Such terminators will usually cause transcription to terminate on both the forward and reverse strand.  Again, however, the efficiency of transcriptional termination is not 100% and will often differ in the forward and reverse direction.
+Finally, there are also reverse transcriptional terminators that terminate transcription on the reverse strand only.
+===Forward terminators===
+<parttable>forward_terminator</parttable>
+<!-- To include a new part in the forward terminator table, make the part type "Terminator" and add the category "//direction/forward" to the part under the Hard Information tab. -->
+===Bidirectional terminators===
+<parttable>bidirectional_terminator</parttable>
+<!-- To include a new part in the forward terminator table, make the part type "Terminator" and add the category "//direction/bidirectional" to the part under the Hard Information tab. -->
+===Reverse terminators===
+<parttable>reverse_terminator</parttable>
+<!-- To include a new part in the forward terminator table, make the part type "Terminator" and add the category "//direction/reverse" to the part under the Hard Information tab. -->
+{|
+|[[Image:ReshmaShettyPhoto.jpg]]
+|Reshma Shetty, as a member of the 2003 MIT IAP class in synthetic biology, designed transcriptional terminators [[Part:BBa_B0011|BBa_B0011]]-[[Part:BBa_B0016|BBa_B0016]].
+|
+|Cassie Huang, as a masters student in Tom Knight's lab, designed, constructed and characterized transcriptional terminators [[Part:BBa_B1001|BBa_B1001]]-[[Part:BBa_B1010|BBa_B1010]].
+|-
+|[[Image:CaitlinConboyPhoto.jpg|60px]]
+|Caitlin Conboy, as a member of Drew Endy's lab, constructed and characterized transcriptional terminators [[Part:BBa_B0021|BBa_B0021]]-[[Part:BBa_B0025|BBa_B0025]] and characterized transcriptional terminators [[Part:BBa_B0011|BBa_B0011]]-[[Part:BBa_B0015|BBa_B0015]].
+|[[Image:JCA Photo.png|60px|center]]
+|Chris Anderson, a professor of bioengineering at UC Berkeley, designed, constructed and characterized terminator [[Part:BBa_J61048|BBa_J61048]].
+|}
+==Yeast terminators==
+{{:Terminators/Yeast/Overview}}
+<parttable>yeast_terminator</parttable>
+<!-- To include a new part in the forward terminator table, make the part type "Terminator" and add the category "//chassis/eukaryote/yeast" to the part under the Hard Information tab. -->
+{{:Terminators/Yeast/Credit}}
+==Eukaryotic terminators==
+Here are all the eukaryotic, including yeast, terminators available.  As you can see, there are only a handful available, so please design, construct, and characterize new ones and submit them to the Registry!
+<parttable>eukaryotic_terminator</parttable>
+<!-- To include a new part in the forward terminator table, make the part type "Terminator" and add the category "//chassis/eukaryote" to the part under the Hard Information tab. -->
+{|
+|[[Image:MonikaCiglicPhoto.jpg|60px|center]]
+|Monika Ciglic constructed this eukaryotic terminator as a member of the [http://2006.igem.org/Ljubljana,_Slovenia_2006 2006 Slovenija iGEM team].
+|}
+_ __NOEDITSECTION__