Part:BBa_K4947020

negative_regulators -NA-

Yeast terminator with I-SceI restriction site This is the terminator used for termination of gene transcription, used as the terminator for each gene in the biosynthetic pathway of daidzein, recombinantly expressed in E. coli.

Secondary Structure

Description

This is the terminator used for termination of gene transcription, used as the terminator for each gene in the biosynthetic pathway of daidzein, recombinantly expressed in E. coli. This part was designed by Jaymin Patel in Farren Isaacs’ lab at Yale University to study cross-kingdom expression. This part was designed with a I-SceI restriction site to be flexible in placement.

Usage

This part was used in the assembly of the biosynthetic pathway of daidzein. In the construct, it followed all CDS’s of the genes used. This part, along with the rest of the biosynthetic pathway of daidzein, was successfully constructed, cloned into, and integrated into the genome of E. coli DH10Beta.

Experience

This part had little to no difficulty in amplification. It also was an effective terminator.

References

1. Cross-kingdom expression of synthetic genetic elements promotes discovery of metabolites in the human microbiome. Patel JR, Oh J, Wang S, Crawford JM, Isaacs FJ. Cell. 2022 Apr 28;185(9):1487-1505.e14. doi: 10.1016/j.cell.2022.03.008. Epub 2022 Apr 1. 10.1016/j.cell.2022.03.008 PubMed 35366417

---

Measurement

• [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]