Terminators/Catalog

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Forward terminators Bidirectional terminators Reverse terminators Yeast terminators Eukaryotic terminators Help!


E. coli terminators

There are several E. coli transcriptional terminators available via the Registry. The most commonly used type of terminator is a forward terminator. When placed downstream of a genetic part that is usually transcribed, a forward transcriptional terminator will cause transcription to abort. However, in general, most transcriptional terminators will not terminate transcription with 100% efficiency. Some RNA polymerases will continue transcribing "through" a transcriptional terminator.

There are also bidirectional transcriptional terminators. Such terminators will usually cause transcription to terminate on both the forward and reverse strand. Again, however, the efficiency of transcriptional termination is not 100% and will often differ in the forward and reverse direction.

Finally, there are also reverse transcriptional terminators that terminate transcription on the reverse strand only.

Forward terminators


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NameDescriptionDirectionEfficiency
Fwd. Rev.		ChassisLength
BBa_B0010T1 from E. coli rrnBForward   80
BBa_B0012TE from coliphageT7Forward0.309[CC]-0.368[CC] 41
BBa_B0013TE from coliphage T7 (+/-)Forward0.6[CC]-1.06[CC] 47
BBa_B0015double terminator (B0010-B0012)Forward0.984[CC]
0.97[JK]		0.295[CC]
0.62[JK]		E.coli129
BBa_B0017double terminator (B0010-B0010)Forward   168
BBa_B0053Terminator (His)Forward   72
BBa_B0055 -- No description --   78
BBa_B1002Terminator (artificial, small, %T~=85%)Forward0.98[CH]  34
BBa_B1003Terminator (artificial, small, %T~=80)Forward0.83[CH]  34
BBa_B1004Terminator (artificial, small, %T~=55)Forward0.93[CH]  34
BBa_B1005Terminator (artificial, small, %T~=25%Forward0.86[CH]  34
BBa_B1006Terminator (artificial, large, %T~>90)Forward0.99[CH]  39
BBa_B1010Terninator (artificial, large, %T~<10)Forward0.95[CH]  40
BBa_I11013Modification of biobricks part BBa_B0015   129
BBa_I51003 -- No description --   110
BBa_J61048[rnpB-T1] TerminatorForward0.98[JCA]  113
BBa_K1392970Terminator+Tetr Promoter+T4 Endolysin   623
BBa_K1486001Arabinose promoter + CpxRForward  Escherichia coli1924
BBa_K1486005Arabinose promoter + sfGFP-CpxR [Cterm]Forward  Escherichia coli2668
BBa_K1486009CxpR & Split IFP1.4 [Nterm + Nterm]Forward  Escherichia coli3726
BBa_K780000Terminator for Bacillus subtilis   54
BBa_K864501T22, P22 late terminatorForward   42
BBa_K864600T0 (21 imm) transcriptional terminatorForward0.97  52
BBa_K864601Lambda t1 transcriptional terminatorForward0.97  53


Bidirectional terminators


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NameDescriptionDirectionEfficiency
Fwd. Rev.		ChassisLength
BBa_B0011LuxICDABEG (+/-)Bidirectional0.419[CC]/0.95[JK]0.636[CC]/0.86[JK] 46
BBa_B0014double terminator (B0012-B0011)Bidirectional0.604[CC]/0.96[JK]0.86[JK] 95
BBa_B0021LuxICDABEG (+/-), reversedBidirectional0.636[CC]/0.86[JK]0.419[CC]/0.95[JK] 46
BBa_B0024double terminator (B0012-B0011), reversedBidirectional0.86[JK]0.604[CC]/0.96[JK] 95
BBa_B0050Terminator (pBR322, +/-)Bidirectional   33
BBa_B0051Terminator (yciA/tonA, +/-)Bidirectional   35
BBa_B1001Terminator (artifical, small, %T~=90)Bidirectional0.81[CH]  34
BBa_B1007Terminator (artificial, large, %T~=80)Bidirectional0.83[CH]  40
BBa_B1008Terminator (artificial, large,  %T~=70)Bidirectional   40
BBa_B1009Terminator (artificial, large, %T~=40%)Bidirectional   40
BBa_K187025terminator in pAB, BioBytes plasmid   60
BBa_K259006GFP-TerminatorBidirectional0.604[CC]/0.96[JK]0.86[JK] 823


Reverse terminators


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NameDescriptionDirectionEfficiency
Fwd. Rev.		ChassisLength
BBa_B0020Terminator (Reverse B0010)Reverse   82
BBa_B0022TE from coliphageT7, reversedReverse-0.368[CC]0.309[CC] 41
BBa_B0023TE from coliphage T7, reversedReverse-1.06[CC]0.6[CC] 47
BBa_B0025double terminator (B0015), reversedReverse0.295[CC]/0.62[JK]0.984[CC]/0.97[JK] 129
BBa_B0052Terminator (rrnC)Forward   41
BBa_B0060Terminator (Reverse B0050)Bidirectional   33
BBa_B0061Terminator (Reverse B0051)Bidirectional   35
BBa_B0063Terminator (Reverse B0053)Reverse   72


ReshmaShettyPhoto.jpg Reshma Shetty, as a member of the 2003 MIT IAP class in synthetic biology, designed transcriptional terminators BBa_B0011-BBa_B0016. Cassie Huang, as a masters student in Tom Knight's lab, designed, constructed and characterized transcriptional terminators BBa_B1001-BBa_B1010.
CaitlinConboyPhoto.jpg Caitlin Conboy, as a member of Drew Endy's lab, constructed and characterized transcriptional terminators BBa_B0021-BBa_B0025 and characterized transcriptional terminators BBa_B0011-BBa_B0015.
JCA Photo.png
 Chris Anderson, a professor of bioengineering at UC Berkeley, designed, constructed and characterized terminator BBa_J61048.


Yeast terminators

Here are all the yeast terminators available. As you can see, there are only a couple available, so please design, construct, and characterize new ones and submit them to the Registry!


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NameDescriptionDirectionEfficiency
Fwd. Rev.		ChassisLength
BBa_J63002ADH1 terminator from S. cerevisiaeForward   225
BBa_K110012STE2 terminatorForward   123
BBa_K1462070cyc1   250
BBa_K1486025ADH1 TerminatorForward  Saccharomyces Cerevisiae188
BBa_K2314608Tmini is a very short terminator in yeast. It's only 68bp in length but has a good performance.   68
BBa_K2637012ADH1 Terminator  S. cerevisiae335
BBa_K2637014TEF1 Terminator  S. cerevisiae476
BBa_K2637016PGK1 Terminator  S. cerevisiae285
BBa_K2637017CYC1 terminator   261
BBa_K3768004CPS1 Terminator for S. cerevisiae   191
BBa_K392003yeast ADH1 terminator   129
BBa_K4947020Yeast terminator with I-SceI restriction site   50
BBa_K801011TEF1 yeast terminator   507
BBa_K801012ADH1 yeast terminator   349
BBa_Y1015CycE1   252


CarolineAjoFranklingPhoto.jpg
 Caroline Ajo-Franklin developed the yeast terminator BBa_J63002 as a post-doc in Pam Silver's lab.


Eukaryotic terminators

Here are all the eukaryotic, including yeast, terminators available. As you can see, there are only a handful available, so please design, construct, and characterize new ones and submit them to the Registry!


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NameDescriptionDirectionEfficiency
Fwd. Rev.		ChassisLength
BBa_J52016eukaryotic -- derived from SV40 early poly A signal sequenceForward   238
BBa_J63002ADH1 terminator from S. cerevisiaeForward   225
BBa_K110012STE2 terminatorForward   123
BBa_K115930735S Terminator of Cauliflower Mosaic Virus (CaMV)   217
BBa_K1462070cyc1   250
BBa_K1484215nopaline synthase terminator   293
BBa_K1486025ADH1 TerminatorForward  Saccharomyces Cerevisiae188
BBa_K2314608Tmini is a very short terminator in yeast. It's only 68bp in length but has a good performance.   68
BBa_K2637012ADH1 Terminator  S. cerevisiae335
BBa_K2637014TEF1 Terminator  S. cerevisiae476
BBa_K2637016PGK1 Terminator  S. cerevisiae285
BBa_K3758200psbA 3-UTR (Nicotiana tabacum)   93
BBa_K392003yeast ADH1 terminator   129
BBa_K404108hGH terminator   481
BBa_K404116hGH_[AAV2]-right-ITR    632
BBa_K4213000Plant Thiamine Pyrophosphate Riboswitch   675
BBa_K4235020SV40 poly(A) signal  Spodoptera Frugiperda135
BBa_K4947020Yeast terminator with I-SceI restriction site   50
BBa_K678012SV40 poly A, terminator for mammalian cells   139
BBa_K678018hGH poly A, terminator for mammalian cells   635
BBa_K678019BGH poly A, mammalian terminator   233
BBa_K678036trpC terminator for Aspergillus nidulans   759
BBa_K678037T1-motni, terminator for Aspergillus niger   1006
BBa_K678038T2-motni, terminator for Aspergillus niger   990
BBa_K678039T3-motni, terminator for Aspergillus niger   889
BBa_K801011TEF1 yeast terminator   507
BBa_K801012ADH1 yeast terminator   349
BBa_Y1015CycE1   252


MonikaCiglicPhoto.jpg
 Monika Ciglic constructed this eukaryotic terminator as a member of the [http://2006.igem.org/Ljubljana,_Slovenia_2006 2006 Slovenija iGEM team].