Part:BBa_K1486025

biology -NA- chassis Saccharomyces Cerevisiae direction Forward function Terminator

ADH1 Terminator The ADH1 terminator (Alcohol dehydrogenase) is a constitutive terminator in the budding yeast S.cerevisae.

Purpose and origin of the Biobrick

The ADH1 terminator was extracted by PCR from plasmids we ordered to addgene:Plasmid 44901 pFA6a-link-yoSuperfolderGFP-Kan and Plasmid 44873 pFA6a-link-yoSuperfolderGFP-CaURA3. A transcription terminator is necessary to avoid mRNA synthesis further than the gene of interest and control which sequence will serve as a 3'UTR, a regulatory subunit of the mRNA.

Secondary Structure

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

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Measurement

• [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]