- The Registry divides a plasmid into two separate components:
- the part
- the plasmid backbone
Prefix and Suffix
- The Prefix and Suffix flank your part
- Your part does not include the prefix and suffix
- The sequence of the plasmid backbone starts with the suffix and ends with the prefix
- Responsible for replicating plasmids during cell growth and division.
- the plasmid copy number per cell
- mechanism of copy number control
- cell-to-cell copy number variation
- ...and even the degree of coiling of the physical DNA.
- Operate your part in the plasmid backbone that works for your system
- Maybe your part uses a lot of cellular resources, so you'll want to operate it in a low copy plasmid backbone
- Or you want to test your part in different organisms by using a multi-host plasmid backbone
VF2 and VR sites
- Use a plasmid backbone to measure your part
- You may want to use a plasmid backbone that will help you measure attributes of your part
- Have a library of promoters you want to test? Use a plasmid backbone with a built in coding region.
- Don't forget, documenting and characterizing your part is important!
Learn More About:
Parts | Plasmid Backbones | BioBrick Prefix and Suffix | Standards | Assembly Standards | Assembly Methods