Group:iGEM18 BostonU

Welcome!

This is the Team BostonU 2018 (AKA "Project Leo") registry page. It is mostly for pointing to other things, if you somehow got here.

Here is our wiki!

It should be noted that all of our parts were designed for use in S. cerevisiae.

Parts List

• BBa_K2805002: (C120op)x5-minCyc1-mRuby2-tCyc1

This is an mRuby reporter driven by a LOV2-compatible blue light-inducible promoter.

• BBa_K2805003: LOV2: NLS-VP16-EL222

This is a gene for expressing LOV2, a blue light-inducible transcription activator that binds to C120op sites (such as the promoter in BBa_K2805002).

• BBa_K2805004: LOV2 -> mRuby

This is a composite of the two parts above, BBa_K2805002 and BBa_K2805003. When integrated into a microbe, that microbe will express a Red Fluorescent Protein when bathed in blue (450 nm) light.

• BBa_K2805005: PhyBNT-Zif268

This part is a red light-inducible transcription activator for the PhiReX system. It will not work without the addition of chromophores PCB and PΦB, which are not native to S. cerevisiae. You can find a heme oxygenase for making these chromphores [[1]] and a ferredoxin oxidoreductase for making these chromophores [[2]].

• BBa_K2805006: PIF3-NLS-VP16

The helix-loop-helix protein PIF3 linked to an NLS and the VP16 transcriptional activator. This is piece of the PhiReX system which dimerizes with PhyBNT when PhyBNT is induced by red (650 nm wavelength) light.

• BBa_K2805007: pGal1(Zif268op)x6-mRuby2

This is an mRuby red fluorescent protein driven by a mutated pGal1 promoter with 6 Zif268 zinc finger binding sites. This part is mean to be used in conjunction with BBa_K2805005 and BBa_K2805006. When used with BBa_K2805005 and BBa_K2805006, the mRuby will report under red (650 nm wavelength) light.

• BBa_K2805008: PhiReX -> mRuby

A composite of BBa_K2805005, BBa_K2805006 and BBa_K2805007.

• BBa_K2805009: HY1

A heme oxygenase enzyme for non-natively expressing the chromophores required for PhyBNT-Zif268 to work.

• BBa_K2805010: {pPGK1-HY1}-{pTEF1-PcyA}

All of the enzymes needed for non-natively expressing the PhiReX system chromophores.

• BBa_K2805012: (C120op)x5-vioC

LOV2-binding promoter driving vioC. For more information on vioC, see iGEM 2016 Washington's registry page on the part.

• BBa_K2805013: LOV2 -> vioC

LOV2 blue light-inducible promoter system for toggling expression of vioC. Composite of BBa_K2805003: LOV2: NLS-VP16-EL222 and BBa_K2805012: (C120op)x5-vioC.